BMPRIA / ALK-3 / CD292 recombinant protein

Recombinant Human BMPRIA / ALK-3 / CD292 Protein (His & Fc tag)

Cat. Num. AAA2546254

• Gene Names: BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: BMPRIA / ALK-3 / CD292; Recombinant Human BMPRIA / ALK-3 / CD292 Protein (His & Fc tag); ACVRLK3; ALK3; CD292; BMPR1A; BMPRIA / ALK-3 / CD292 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 532

• Application Notes: The recombinant human ALK3/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 376 amino acids and has a predicted molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh ALK3/Fc monomer is approximately 56 kDa due to glycosylation.
• Predicted N Terminal: Gln 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BMPRIA / ALK-3 / CD292 recombinant protein

Background: Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-beta) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.

Description: A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of human ALK3 (NP_004320.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 41349437
• NCBI GeneID: 657
• NCBI Accession #: NP_004320.2
• NCBI GenBank Nucleotide #: NM_004329.2
• UniProt Accession #: P36894; Q8NEN8; A8K6U9
• Molecular Weight: 60,198 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor type 1A
• NCBI Official Symbol: BMPR1A
• NCBI Official Synonym Symbols: ALK3; SKR5; CD292; ACVRLK3; 10q23del
• NCBI Protein Information: bone morphogenetic protein receptor type-1A
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• UniProt Gene Name: BMPR1A
• UniProt Synonym Gene Names: ACVRLK3; ALK3; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5

NCBI Description

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 10q23.2

Cellular Component: caveola; external side of plasma membrane; plasma membrane

Molecular Function: ATP binding; glycoprotein binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; SMAD binding; transmembrane receptor protein serine/threonine kinase activity

Biological Process: BMP signaling pathway; immune response; negative regulation of smooth muscle cell migration; positive regulation of bone mineralization; positive regulation of cardiac muscle cell proliferation; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cardiac muscle cell proliferation; transforming growth factor beta receptor signaling pathway

Disease: Juvenile Polyposis Syndrome; Polyposis Syndrome, Hereditary Mixed, 2