BMPR1A/ALK-3 Active Protein | BMPR1A active protein

BMPR1A/ALK-3 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122522

• Gene Names: Bmpr1a; ALK3; Bmpr; BMPR-IA; AU045487; 1110037I22Rik
• Purity: >97% as determined by SDS-PAGE
• Synonyms: BMPR1A/ALK-3; BMPR1A/ALK-3 Protein; Mouse; Recombinant (His Tag); Mouse ALK-3/BMPR1A Protein (His Tag); 1110037I22Rik Protein; ALK3 Protein; AU045487 Protein; Bmpr Protein; BMPR-IA Protein; bone morphogenetic protein receptor; type IA; BMPR1A active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg152

• Species: Mouse
• Activity: Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2ug/ml in the presence of 50ng/mL of recombinant human BMP4.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Arg 152) of mouse ALK3 (NP_033888.2) precursor was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 ug/ml in the presence of 50 ng/ml of recombinant human BMP4.)

Related Product Information for BMPR1A active protein

Background: Activin receptor-Like Kinase 3 (ALK-3), also known as Bone Morphogenetic Protein Receptor, type IA (BMPR1A), is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the transforming growth factor beta (TGF-beta) superfamily. The BMP receptors form a subfamily of transmembrane serine/threonine kinases including the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. ALK-3/BMPR1A is expressed in the epithelium during branching morphogenesis. Deletion of BMPR1A in the epithelium with an Sftpc-cre transgene leads to dramatic defects in lung development. ALK-3 and ALK-6 share a high degree of homology, yet possess distinct signaling roles. The transforming growth factor (TGF)-beta type III receptor (TbetaRIII) enhanced both ALK-3 and ALK-6 signaling. TbetaRIII associated with ALK-3 primarily through their extracellular domains, whereas its interaction with ALK-6 required both the extracellular and cytoplasmic domains. ALK-3 plays an essential role in the formation of embryonic ventral abdominal wall, and abrogation of BMP signaling activity due to gene mutations in its signaling components could be one of the underlying causes of omphalocele at birth. The type IA BMP receptor, ALK-3 was specifically required at mid-gestation for normal development of the trabeculae, compact myocardium, interventricular septum, and endocardial cushion. Cardiac muscle lacking ALK-3 was specifically deficient in expressing TGFbeta2, an established paracrine mediator of cushion morphogenesis. Hence, ALK-3 is essential, beyond just the egg cylinder stage, for myocyte-dependent functions and signals in cardiac organogenesis.

References

Gaussin V, et al. (2002) Endocardial cushion and myocardial defects after cardiac myocyte-specific conditional deletion of the bone morphogenetic protein receptor ALK3. Proc Natl Acad Sci U S A. 99(5): 2878-83.Eblaghie MC, et al. (2006) Evidence that autocrine signaling through Bmpr1a regulates the proliferation, survival and morphogenetic behavior of distal lung epithelial cells. Dev Biol. 291(1): 67-82.Sun J, et al. (2007) Deficient Alk3-mediated BMP signaling causes prenatal omphalocele-like defect. Biochem Biophys Res Commun. 360(1): 238-43.Lee NY, et al. (2009) The transforming growth factor-beta type III receptor mediates distinct subcellular trafficking and downstream signaling of activin-like kinase (ALK)3 and ALK6 receptors. Mol Biol Cell. 20(20): 4362-70.

NCBI and Uniprot Product Information

• NCBI GI #: 46519168
• NCBI GeneID: 12166
• NCBI Accession #: NP_033888.2
• NCBI GenBank Nucleotide #: NM_009758.4
• UniProt Accession #: P36895
• Molecular Weight: 60,063 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type 1A
• NCBI Official Symbol: Bmpr1a
• NCBI Official Synonym Symbols: ALK3; Bmpr; BMPR-IA; AU045487; 1110037I22Rik
• NCBI Protein Information: bone morphogenetic protein receptor type-1A
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: Bmpr1a
• UniProt Synonym Gene Names: Acvrlk3; Bmpr; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5
• UniProt Entry Name: BMR1A_MOUSE

Uniprot Description

BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: Membrane protein, integral; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; Protein kinase, TKL; Kinase, protein; TKL group; STKR family; Type1 subfamily

Cellular Component: cell soma; membrane; dendrite; plasma membrane; integral to membrane; caveola

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein homodimerization activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: neural plate mediolateral pattern formation; transcription from RNA polymerase II promoter; paraxial mesoderm development; heart morphogenesis; hindlimb morphogenesis; developmental growth; neural crest cell development; positive regulation of transcription, DNA-dependent; heart development; mesendoderm development; paraxial mesoderm structural organization; dorsal/ventral axis specification; palate development; protein amino acid phosphorylation; negative regulation of neurogenesis; BMP signaling pathway; anterior/posterior pattern formation; transmembrane receptor protein serine/threonine kinase signaling pathway; positive regulation of mesenchymal cell proliferation; ectoderm development; embryonic morphogenesis; cell differentiation; Mullerian duct regression; nervous system development; regulation of endothelial cell proliferation; somitogenesis; in utero embryonic development; lateral mesoderm development; stem cell maintenance; positive regulation of bone mineralization; pattern specification process; regulation of endothelial cell differentiation; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; mesoderm formation; dorsal/ventral pattern formation; pituitary gland development; cartilage development; embryonic organ development; immune response; embryonic digit morphogenesis; phosphorylation; regulation of lateral mesodermal cell fate specification; positive regulation of epithelial cell proliferation; lung development; endoderm development