Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-Like Kinase 3 Active Protein | Bmpr1a active protein

Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-Like Kinase 3

Cat. Num. AAA2556804

• Gene Names: Bmpr1a; ALK3; Bmpr; SKR5; ALK-3; BMPR-1A; BMPR-IA; AU045487; 1110037I22Rik
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-Like Kinase 3; Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-Like Kinase 3; ALK-3; Bone morphogenetic protein receptor type-1A; BMP type-1A receptor; BMPR-1A; Activin receptor-like kinase 3; BMP-2/BMP-4 receptor; Serine/threonine-protein kinase receptor R5; SKR5; CD292; ; Acvrlk3; Bmpr1a active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Length: 532

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Gln24-Arg152
• Fusion Tag: C-Fc-6His
• Activity: Measured by its ability to inhibit recombinant human BMP4 induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.5-2 mug/ml in the presence of 50 ng/ml of recombinant human BMP4.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-Page

Related Product Information for Bmpr1a active protein

Protein Construction: Recombinant Mouse Bone Morphogenetic Protein Receptor Type IA/Activin Receptor-like Kinase 3 is produced by our Mammalian expression system and the target gene encoding Gln24-Arg152 is expressed with a Fc, 6His tag at the C-terminus.

Background: ALK-3 is a type I receptor for bone morphogenetic proteins (BMPs) which belong to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. The BMP receptors consists of the type I receptors BMPR1A and BMPR1B and the type I I receptor BMPR2. Seven known type I serine/threonine kinases and five mammalian type II serine/threonine kinase receptors function in TGF-beta superfamily signal transduction. The downstream molecules of the type I BMP receptors include the Smad (Smad1, 5 and 8) proteins that are phosphorylated in a ligand-dependent manner, and relay the BMP signal from the receptors to target genes in the nucleus. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. ALK-3 contains a GS domain and a protein kinase domain. ALK-3 is widely expressed. Defects in BMPR1A gene are a cause of a significant proportion of cases of Juvenile polyposis syndrome (JPS).

NCBI and Uniprot Product Information

• NCBI GI #: 46519168
• NCBI GeneID: 12166
• NCBI Accession #: NP_033888.2
• NCBI GenBank Nucleotide #: NM_009758.4
• UniProt Accession #: P36895
• Molecular Weight: Molecular Mass: 42.2kDa; Actual Protein Molecular Mass: 55-60 & 120kDa
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type 1A
• NCBI Official Symbol: Bmpr1a
• NCBI Official Synonym Symbols: ALK3; Bmpr; SKR5; ALK-3; BMPR-1A; BMPR-IA; AU045487; 1110037I22Rik
• NCBI Protein Information: bone morphogenetic protein receptor type-1A
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: Bmpr1a
• UniProt Synonym Gene Names: Acvrlk3; Bmpr; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5

Uniprot Description

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction (PubMed:24098149). Mediates induction of adipogenesis by GDF6 (PubMed:23527555).