Western Blot (WB)
((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PTCHD3 Polyclonal Antibody | anti-PTCHD3 antibody
Goat anti-PTCHD3 Antibody
Gene Names
PTCHD3; PTR
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PTCHD3; Polyclonal Antibody; Goat anti-PTCHD3 Antibody; patched domain containing 3; FLJ44037; MGC129888; PTR; patched domain-containing protein 3; patched-related protein; PTCHD3 antibody; patched domain containing 3 antibody; FLJ44037 antibody; MGC129888 antibody; PTR antibody; patched domain-containing protein 3 antibody; patched-related protein antibody; anti-PTCHD3 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KKVDYWDKDVRQ
Sequence Length
767
Applicable Applications for anti-PTCHD3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.9kDa according to NP_001030014.2). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.9kDa according to NP_001030014.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-KKVDYWDKDVRQ, from the internal region of the protein sequence according to NP_001030014.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,872 Da
NCBI Official Full Name
patched domain-containing protein 3
NCBI Official Synonym Full Names
patched domain containing 3
NCBI Official Symbol
PTCHD3
NCBI Official Synonym Symbols
PTR
NCBI Protein Information
patched domain-containing protein 3
UniProt Protein Name
Patched domain-containing protein 3
Protein Family
UniProt Gene Name
PTCHD3
UniProt Synonym Gene Names
PTR
UniProt Entry Name
PTHD3_HUMAN
Uniprot Description
PTCHD3: May play a role in sperm development or sperm function. Belongs to the patched family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 10p12.1
Cellular Component: integral to membrane
Molecular Function: hedgehog receptor activity
Biological Process: signal transduction; spermatid development
Research Articles on PTCHD3
Similar Products
Product Notes
The PTCHD3 ptchd3 (Catalog #AAA423061) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PTCHD3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTCHD3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.9kDa according to NP_001030014.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PTCHD3 ptchd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KKVDYWDKDV RQ. It is sometimes possible for the material contained within the vial of "PTCHD3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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