Human bone morphogenetic protein 8b ELISA Kit | BMP8B elisa kit

Human Bone morphogenetic protein 8B, BMP8B ELISA Kit

Cat. Num. AAA944757

• Gene Names: BMP8B; OP2; BMP8
• Reactivity: Human
• Synonyms: bone morphogenetic protein 8b; Human Bone morphogenetic protein 8B; BMP8B ELISA Kit; Human Bone morphogenetic protein 8B (BMP8B) ELISA kit; BMP8; MGC131757; OP2; bone morphogenetic protein 8 (osteogenic protein 2) ; bone morphogenetic protein 8B; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2) ) ; osteogenic protein 2; BMP8B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human BMP8B. No significant cross-reactivity or interference between human BMP8B and analogues was observed.
• Sequence Length: 402

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 9.37 pg/ml-600 pg/ml
• Sensitivity: < 2.34 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP8B elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP8B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP8B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP8B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP8B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 29571106
• NCBI GeneID: 656
• NCBI Accession #: NP_001711.2
• NCBI GenBank Nucleotide #: NM_001720.3
• UniProt Accession #: P34820; Q53ZM7; Q9NUF0
• Molecular Weight: 44,768 Da
• NCBI Official Full Name: bone morphogenetic protein 8B preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 8b
• NCBI Official Symbol: BMP8B
• NCBI Official Synonym Symbols: OP2; BMP8
• NCBI Protein Information: bone morphogenetic protein 8B; BMP-8; BMP-8B; osteogenic protein 2; bone morphogenetic protein 8 (osteogenic protein 2); dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2))
• UniProt Protein Name: Bone morphogenetic protein 8B
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP8B
• UniProt Synonym Gene Names: BMP8; BMP-8; BMP-8B; OP-2
• UniProt Entry Name: BMP8B_HUMAN

NCBI Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP8B: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Belongs to the TGF-beta family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p35-p32

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; BMP signaling pathway; ossification; regulation of MAPKKK cascade; cartilage development; skeletal development; cell development; growth