Rat Bone Morphogenetic Protein 3 ELISA Kit | BMP-3 elisa kit

Rat Bone Morphogenetic Protein 3 ELISA Kit

Cat. Num. AAA762419

• Gene Names: BMP3; BMP-3A
• Reactivity: Rat
• Synonyms: Bone Morphogenetic Protein 3; Rat Bone Morphogenetic Protein 3 ELISA Kit; BMP-3 (Bone Morphogenetic Protein 3)/Osteogenin/BMP3A/BMP-3A/bone morphogenetic protein 3/Bone morphogenetic protein 3A; BMP-3 elisa kit

• Reactivity: Rat
• Sequence Length: 472

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BMP-3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat BMP-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat BMP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat BMP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat BMP-3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 126507087
• NCBI GeneID: 651
• NCBI Accession #: NP_001192.2
• NCBI GenBank Nucleotide #: NM_001201.2
• Molecular Weight: 53,372 Da
• NCBI Official Full Name: bone morphogenetic protein 3 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 3
• NCBI Official Symbol: BMP3
• NCBI Official Synonym Symbols: BMP-3A
• NCBI Protein Information: bone morphogenetic protein 3
• UniProt Protein Name: Bone morphogenetic protein 3
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP3
• UniProt Synonym Gene Names: BMP3A; BMP-3; BMP-3A
• UniProt Entry Name: BMP3_HUMAN

NCBI Description

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein, also known as osteogenin, induces bone formation. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP3: Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; receptor binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cartilage development; cell development; cell-cell signaling; growth; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of MAPKKK cascade; skeletal development