Testing Data
Alkaline phosphatase Recombinant Protein | ALPI recombinant protein
Alkaline phosphatase Protein, Human, Recombinant (hFc Tag)
Gene Names
ALPI; IAP
Purity
>83 % as determined by SDS-PAGE
Synonyms
Alkaline phosphatase; Alkaline phosphatase Protein; Human; Recombinant (hFc Tag); Human Alkaline Phosphatase/ALPI Protein (Fc Tag); alkaline phosphatase; intestinal; IAP Protein; ALPI recombinant protein
Host
HEK293 Cells
Purity/Purification
>83 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4 Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence Positions
725aa
Sequence
Met1-Asp503
Species
Human
Tag
hFc
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Val20
Protein Construction
|A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503), without the pro peptide, was fused with a polyhistidine tag at the C-terminus
Activity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).
The specific activity is >10, 000 pmoles/min/ug.
The specific activity is >10, 000 pmoles/min/ug.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping: They are shipped out with blue ice pack
Shipping: They are shipped out with blue ice pack
Testing Data
Testing Data
Related Product Information for ALPI recombinant protein
ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Product Categories/Family for ALPI recombinant protein
References
Nakano T, et al. (2009) Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. Am J Physiol Gastrointest Liver Physiol. 297(1): G207-14.Henthorn PS, et al. (1987) Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase. Proc Natl Acad Sci. 84(5): 1234-8.Oetting WS, et al. (2003) Oculocutaneous albinism type 1: the last 100 years. Pigment Cell Res. 16(3): 307-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,812 Da
NCBI Official Full Name
intestinal-type alkaline phosphatase
NCBI Official Synonym Full Names
alkaline phosphatase, intestinal
NCBI Official Symbol
ALPI
NCBI Official Synonym Symbols
IAP
NCBI Protein Information
intestinal-type alkaline phosphatase; Kasahara isozyme; alkaline phosphomonoesterase; glycerophosphatase; intestinal alkaline phosphatase
UniProt Protein Name
Intestinal-type alkaline phosphatase
Protein Family
UniProt Gene Name
ALPI
UniProt Entry Name
PPBI_HUMAN
NCBI Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]
Research Articles on ALPI
Similar Products
Product Notes
The ALPI alpi (Catalog #AAA8118682) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 725aa. The amino acid sequence is listed below: Met1-Asp50 3. It is sometimes possible for the material contained within the vial of "Alkaline phosphatase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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