WRAP53 blocking peptide

WRAP53 Blocking Peptide

Cat. Num. AAA9621209

• Gene Names: WRAP53; DKCB3; TCAB1; WDR79
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: WRAP53; WRAP53 Blocking Peptide; FLJ10385; TCAB1; Telomerase Cajal body protein 1; WAP53_HUMAN; WD repeat containing protein 79; WD repeat-containing protein 79; WD40 repeat-containing protein encoding RNA antisense to p53; Wrap53; WRAP53 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for WRAP53 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for WRAP53 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 221136858
• NCBI GeneID: 55135
• NCBI Accession #: NP_001137462.1
• NCBI GenBank Nucleotide #: NM_001143990.1
• UniProt Accession #: Q9BUR4; Q08ET9; Q9NW09; B3KPR9; D3DTQ4
• Molecular Weight: 59,309 Da
• NCBI Official Full Name: telomerase Cajal body protein 1
• NCBI Official Synonym Full Names: WD repeat containing, antisense to TP53
• NCBI Official Symbol: WRAP53
• NCBI Official Synonym Symbols: DKCB3; TCAB1; WDR79
• NCBI Protein Information: telomerase Cajal body protein 1; WD40 protein Wrap53; WD repeat-containing protein 79; WD40 repeat-containing protein encoding RNA antisense to p53
• UniProt Protein Name: Telomerase Cajal body protein 1
• Protein Family: Telomerase Cajal body protein
• UniProt Gene Name: WRAP53
• UniProt Synonym Gene Names: TCAB1; WDR79
• UniProt Entry Name: WAP53_HUMAN

Uniprot Description

Function: Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage. Ref.11 Ref.13

Subunit structure: Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC). Ref.13

Subcellular location: Nucleus › Cajal body. Cytoplasm Ref.11 Ref.13.

Tissue specificity: Expressed in all tissues and cell lines examined. Ref.11

Involvement in disease: Dyskeratosis congenita, autosomal recessive, 3 (DKCB3) [MIM:613988]: A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.16

Sequence similarities: Contains 6 WD repeats.