Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: HT-29, hela and A431 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Rabbit anti-Human WRAP53 Polyclonal Antibody | anti-WRAP53 antibody
WRAP53 Antibody
Gene Names
WRAP53; DKCB3; TCAB1; WDR79
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
WRAP53; Polyclonal Antibody; WRAP53 Antibody; DKCB3; TCAB1; WDR79; anti-WRAP53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total WRAP53 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
548
Applicable Applications for anti-WRAP53 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:10-1:50
Immunohistochemistry: 1:10-1:50
Immunogen
Fusion protein of human WRAP53
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: HT-29, hela and A431 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Western Blot (WB)
(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: HT-29, hela and A431 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/20,)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/20,)
Related Product Information for anti-WRAP53 antibody
This gene encodes an essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies, nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin, TERT and TERC, other components of active telomerase, and with small Cajal body RNAs (scaRNAs), which are involved in modifying splicing RNAs.
Product Categories/Family for anti-WRAP53 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,309 Da
NCBI Official Full Name
telomerase Cajal body protein 1
NCBI Official Synonym Full Names
WD repeat containing, antisense to TP53
NCBI Official Symbol
WRAP53
NCBI Official Synonym Symbols
DKCB3; TCAB1; WDR79
NCBI Protein Information
telomerase Cajal body protein 1
UniProt Protein Name
Telomerase Cajal body protein 1
Protein Family
UniProt Gene Name
WRAP53
UniProt Synonym Gene Names
TCAB1; WDR79
UniProt Entry Name
WAP53_HUMAN
Uniprot Description
WRAP53: a protein of unknown function. Contains 6 WD40 domains, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: Cajal body; cytoplasm; nucleus; telomerase holoenzyme complex
Molecular Function: chaperone binding; protein binding; protein complex binding; RNA binding
Biological Process: positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; telomere formation via telomerase; telomere maintenance via telomerase
Disease: Dyskeratosis Congenita, Autosomal Recessive, 3
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Product Notes
The WRAP53 wrap53 (Catalog #AAA9416279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WRAP53 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WRAP53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:10-1:50. Researchers should empirically determine the suitability of the WRAP53 wrap53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WRAP53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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