UBE2W blocking peptide

UBE2W Antibody (C-term) Blocking peptide

• Gene Names: UBE2W; UBC16; UBC-16
• Synonyms: UBE2W; UBE2W Antibody (C-term) Blocking peptide; Ubiquitin-conjugating enzyme E2 W; N-terminus-conjugating E2; Ubiquitin carrier protein W; Ubiquitin-conjugating enzyme 16; UBC-16; Ubiquitin-protein ligase W; UBC16; UBE2W blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of UBE2W. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 151

• Cellular Location: Nucleus. Note: In the nucleus, colocalizes with FANCL
• Tissue Location: Widely expressed, with highest expression in brain, liver, pancreas and heart.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for UBE2W blocking peptide

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.

NCBI and Uniprot Product Information

• NCBI GI #: 74751754
• NCBI GeneID: 55284
• UniProt Accession #: Q96B02; Q1XBE0; Q9H823; Q9HAG6; Q9NV07; B4DIV1
• Molecular Weight: 20,689 Da
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 W
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 W (putative)
• NCBI Official Symbol: UBE2W
• NCBI Official Synonym Symbols: UBC16; UBC-16
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 W
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 W
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2W
• UniProt Synonym Gene Names: UBC16; UBC-16
• UniProt Entry Name: UBE2W_HUMAN

NCBI Description

This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: misfolded or incompletely synthesized protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination