TOP1 blocking peptide

TOP1 Antibody (N-term) Blocking peptide

• Gene Names: TOP1; TOPI
• Synonyms: TOP1; TOP1 Antibody (N-term) Blocking peptide; DNA topoisomerase 1; DNA topoisomerase I; TOP1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of TOP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 765

• Cellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Note: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli
• Tissue Location: Endothelial cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TOP1 blocking peptide

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 12644118
• NCBI GeneID: 7150
• UniProt Accession #: P11387; O43256; Q12855; Q12856; Q15610; Q5TFY3; Q9UJN0; A8KA78; E1P5W3
• Molecular Weight: 90,726 Da
• NCBI Official Full Name: DNA topoisomerase 1
• NCBI Official Synonym Full Names: topoisomerase (DNA) I
• NCBI Official Symbol: TOP1
• NCBI Official Synonym Symbols: TOPI
• NCBI Protein Information: DNA topoisomerase 1
• UniProt Protein Name: DNA topoisomerase 1
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP1
• UniProt Entry Name: TOP1_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.

Protein type: Nucleolus; EC 5.99.1.2; Isomerase; Oncoprotein

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; DNA topoisomerase type I activity; protein binding

Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; protein sumoylation; response to drug