Smarca4 blocking peptide

Smarca4 Blocking Peptide (C-term)

• Gene Names: Smarca4; Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• Synonyms: Smarca4; Smarca4 Blocking Peptide (C-term); Transcription activator BRG1; 364-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Baf190a; Brg1; Snf2b; Snf2l4; Smarca4 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 1352-1366 of HUMAN Smarca4
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1613

• Cellular Location: Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Smarca4 blocking peptide

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron- specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 123790047
• NCBI GeneID: 20586
• UniProt Accession #: Q3TKT4; Q3TUD7; Q6AXG8
• Molecular Weight: 181,514 Da
• NCBI Official Full Name: Transcription activator BRG1
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: Smarca4
• NCBI Official Synonym Symbols: Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• NCBI Protein Information: transcription activator BRG1
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Transcription activator
• UniProt Gene Name: Smarca4
• UniProt Synonym Gene Names: Baf190a; Brg1; Snf2b; Snf2l4; BAF190A
• UniProt Entry Name: SMCA4_MOUSE

Uniprot Description

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Helicase; Nuclear receptor co-regulator; EC 3.6.1.-; EC 3.6.4.-; Tumor suppressor; Cell cycle regulation

Cellular Component: extracellular space; heterochromatin; membrane; nuclear chromatin; nucleolus; nucleus; perichromatin fibrils; protein complex; SWI/SNF complex

Molecular Function: androgen receptor binding; ATPase activity; chromatin binding; DNA-dependent ATPase activity; nucleosomal DNA binding; p53 binding; protein binding; protein complex binding; protein N-terminus binding; Tat protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding

Biological Process: ATP-dependent chromatin remodeling; blastocyst growth; blastocyst hatching; blood vessel development; cell morphogenesis; chromatin remodeling; cytosine methylation within a CG sequence; embryonic hindlimb morphogenesis; embryonic organ morphogenesis; epidermis morphogenesis; extracellular matrix organization and biogenesis; forebrain development; glial cell fate determination; heart development; hindbrain development; in utero embryonic development; keratinocyte differentiation; liver development; methylation-dependent chromatin silencing; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; neurogenesis; nucleosome assembly; nucleosome disassembly; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of cell migration; stem cell maintenance; vasculogenesis