Rabbit anti-Human, mouse Smarca4 Polyclonal Antibody | anti-Smarca4 antibody

(Mouse) Smarca4 Antibody (C-term)

Cat. Num. AAA9211370

• Gene Names: Smarca4; Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Smarca4; Polyclonal Antibody; (Mouse) Smarca4 Antibody (C-term); Transcription activator BRG1; 364-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Baf190a; Brg1; Snf2b; Snf2l4; anti-Smarca4 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Smarca4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1352-1386 amino acids from the C-terminal region of mouse Smarca4.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 1617

• Applicable Applications for anti-Smarca4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-Smarca4 Antibody (C-term) at 1:2000 dilutionLane 1: NIH/3T3 whole cell lysatesLane 2: K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 181 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Anti-Smarca4 Antibody (C-term)at 1:2000 dilution + K562 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 181 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Smarca4 antibody

Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron- specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity).

Product Categories/Family for anti-Smarca4 antibody

Crown Antibodies; Stem Cells

References

Carninci P.,et al.Science 309:1559-1563(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lessard J.,et al.Neuron 55:201-215(2007).
Ohkawa Y.,et al.EMBO J. 25:490-501(2006).
Villen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 291463269
• NCBI GeneID: 20586
• NCBI Accession #: NP_001167549.1
• NCBI GenBank Nucleotide #: NM_001174078.1
• UniProt Accession #: Q3TKT4; Q3TUD7; Q6AXG8
• Molecular Weight: 181427
• NCBI Official Full Name: transcription activator BRG1 isoform 1
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: Smarca4
• NCBI Official Synonym Symbols: Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• NCBI Protein Information: transcription activator BRG1
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Transcription activator
• UniProt Gene Name: Smarca4
• UniProt Synonym Gene Names: Baf190a; Brg1; Snf2b; Snf2l4; BAF190A
• UniProt Entry Name: SMCA4_MOUSE

Uniprot Description

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing.

Protein type: Helicase; Transcription, coactivator/corepressor; EC 3.6.4.-; EC 3.6.1.-; Cell cycle regulation; Tumor suppressor; Nuclear receptor co-regulator

Cellular Component: heterochromatin; extracellular space; SWI/SNF complex; protein complex; perichromatin fibrils; membrane; nuclear chromatin; nucleolus; nucleus

Molecular Function: DNA-dependent ATPase activity; nucleosomal DNA binding; histone binding; hydrolase activity; p53 binding; ATPase activity; transcription coactivator activity; nucleotide binding; protein N-terminus binding; helicase activity; transcription factor binding; protein binding; hydrolase activity, acting on acid anhydrides; DNA binding; androgen receptor binding; Tat protein binding; protein complex binding; chromatin binding; transcription corepressor activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: transcription from RNA polymerase II promoter; extracellular matrix organization and biogenesis; glial cell fate determination; heart development; positive regulation of transcription, DNA-dependent; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of cell migration; embryonic organ morphogenesis; regulation of transcription, DNA-dependent; epidermis morphogenesis; blastocyst hatching; positive regulation of cell proliferation; forebrain development; blastocyst growth; vasculogenesis; positive regulation of Wnt receptor signaling pathway; positive regulation of DNA binding; hindbrain development; nervous system development; blood vessel development; transcription, DNA-dependent; in utero embryonic development; stem cell maintenance; chromatin modification; liver development; keratinocyte differentiation; chromatin remodeling; nucleosome assembly; cytosine methylation within a CG sequence; nucleosome disassembly; methylation-dependent chromatin silencing; neurogenesis; ATP-dependent chromatin remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of cell growth; positive regulation of cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis