Rabbit BRG1 Polyclonal Antibody | anti-BRG1 antibody

BRG1 Rabbit pAb

Cat. Num. AAA4756081

• Gene Names: Smarca4; Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: BRG1; Polyclonal Antibody; BRG1 Rabbit pAb; Transcription activator BRG1; SWI/SNF related; matrix associated; actin dependent regulator of chromatin; subfamily a; member 4; Brg1; BAF190A; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo; anti-BRG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-BRG1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: WB: 1:2000-1:10,000; IHC: 1:20-1:100; ICC/IF: 1:50; FC/FACS: 1:20; IP: 1:20-1:50; ChIP: 1:20
• Immunogen: A synthetic peptide of mouse BRG1
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of BRG1 in 293T,Hela,HepG2, A594 cell lysates using BRG1 Rabbit pAb(1:1000 diluted).Predicted band size:185kDa.Observed band size:220kDa.)

Related Product Information for anti-BRG1 antibody

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role in regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (PubMed:26138476). Binds to RNA in a promiscuous manner (PubMed:26138476). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (PubMed:26138476).

Product Categories/Family for anti-BRG1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 291463269
• NCBI GeneID: 20586
• NCBI Accession #: NP_001167549.1
• NCBI GenBank Nucleotide #: NM_001174078.1
• UniProt Accession #: Q3TKT4; Q3TUD7; Q6AXG8
• Molecular Weight: 181,427 Da
• NCBI Official Full Name: transcription activator BRG1 isoform 1
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• NCBI Official Symbol: Smarca4
• NCBI Official Synonym Symbols: Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo
• NCBI Protein Information: transcription activator BRG1; BAF190A; SNF2-beta; protein brahma homolog 1; BRG1-associated factor 190A; ATP-dependent helicase SMARCA4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
• UniProt Protein Name: Transcription activator BRG1
• Protein Family: Biofilm regulator
• UniProt Gene Name: Smarca4
• UniProt Synonym Gene Names: Baf190a; Brg1; Snf2b; Snf2l4; BAF190A
• UniProt Entry Name: SMCA4_MOUSE