RPA2 blocking peptide

RPA2 Blocking Peptide

Cat. Num. AAA9624174

• Gene Names: POLR1B; RPA2; RPA135; Rpo1-2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: RPA2; RPA2 Blocking Peptide; A127; DNA directed RNA polymerase I 135kDa polypeptide; DNA directed RNA polymerase I; DNA directed RNA polymerase I subunit RPA2; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I subunit rpa2; FLJ10816; FLJ21921; MGC131780; OTTHUMP00000161989; OTTHUMP00000203643; OTTHUMP00000203645; POLR 1B; polr1b; Polymerase (RNA) I polypeptide B 128kDa; Polymerase (RNA) I polypeptide B; RNA polymerase I polypeptide B; RNA polymerase I subunit 2; RPA 135; RPA116; RPA135; RPA2_HUMAN; Rpo1 2; RPA2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for RPA2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RPA2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 212286172
• NCBI GeneID: 84172
• NCBI Accession #: NP_001131076.1
• NCBI GenBank Nucleotide #: NM_001137604.2
• UniProt Accession #: Q9H9Y6; Q2TAM4; Q585T5; Q6ZRR2; Q9H9D3; B7Z6Y7; B7Z823; F5GZX4; F8W898
• Molecular Weight: 128,229 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA2 isoform 2
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide B, 128kDa
• NCBI Official Symbol: POLR1B
• NCBI Official Synonym Symbols: RPA2; RPA135; Rpo1-2
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA2; RNA polymerase I subunit 2; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I 135 kDa polypeptide
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA2
• Protein Family: Replication protein
• UniProt Gene Name: POLR1B
• UniProt Synonym Gene Names: RNA polymerase I subunit 2; RPA135
• UniProt Entry Name: RPA2_HUMAN

NCBI Description

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]

Uniprot Description

POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described.

Protein type: Transcription initiation complex; Nucleotide Metabolism - pyrimidine; EC 2.7.7.6; Nucleolus; Nucleotide Metabolism - purine; Transferase

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; ribonucleoside binding; metal ion binding

Biological Process: negative regulation of gene expression, epigenetic; nucleologenesis; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; embryo implantation; rRNA transcription