Rabbit anti-Human GK Polyclonal Antibody | anti-GK antibody

GK, NT (GK, Glycerol kinase, ATP:glycerol 3-phosphotransferase) (Azide free) (HRP)

Cat. Num. AAA6302351

• Gene Names: GK; GK1; GKD
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GK; Polyclonal Antibody; NT (GK; Glycerol kinase; ATP:glycerol 3-phosphotransferase) (Azide free) (HRP); ATP:glycerol 3-phosphotransferase; anti-GK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 530

• Applicable Applications for anti-GK antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human GK.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GK (arrow) using rabbit polyclonal GK Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GK gene.)

Immunohistochemistry (IHC)

(GK Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GK antibody

The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-GK antibody

Antibodies; Protein Kinases

References

Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008). Zhang, Y.H., et al. Pediatr. Res. 59 (4 PT 1), 590-592 (2006): Ohira, R.H., et al. Biochem. Biophys. Res. Commun. 331(1):239-246(2005). Stepanian, S.V., et al. Mol. Genet. Metab. 80(4):412-418(2003). Hellerud, C., et al. Clin. Chem. Lab. Med. 41(1):46-55(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 42794763
• NCBI GeneID: 2710
• NCBI Accession #: NP_976325.1; NP_001121599.1
• NCBI GenBank Nucleotide #: NP_976325.1
• UniProt Accession #: P32189
• NCBI Official Full Name: glycerol kinase isoform a
• NCBI Official Synonym Full Names: glycerol kinase
• NCBI Official Symbol: GK
• NCBI Official Synonym Symbols: GK1; GKD
• NCBI Protein Information: glycerol kinase
• UniProt Protein Name: Glycerol kinase
• Protein Family: Glycerol kinase
• UniProt Gene Name: GK
• UniProt Synonym Gene Names: GK
• UniProt Entry Name: GLPK_HUMAN

NCBI Description

The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Uniprot Description

GLPK: Key enzyme in the regulation of glycerol uptake and metabolism. Defects in GK are the cause of GK deficiency (GKD). This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only. Belongs to the FGGY kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Lipid Metabolism - glycerolipid; EC 2.7.1.30; Kinase, other

Chromosomal Location of Human Ortholog: Xp21.3

Cellular Component: mitochondrial outer membrane; nucleus; cytosol

Molecular Function: glycerol kinase activity; protein binding; ATP binding

Biological Process: triacylglycerol metabolic process; glycerol metabolic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; glucose homeostasis; phosphorylation; glycerol-3-phosphate biosynthetic process; glycerol catabolic process

Disease: Glycerol Kinase Deficiency