Rabbit POLR1B Polyclonal Antibody | anti-POLR1B antibody

POLR1B Antibody

Cat. Num. AAA9402036

• Gene Names: POLR1B; RPA2; RPA135; Rpo1-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: POLR1B; Polyclonal Antibody; POLR1B Antibody; DNA-directed RNA polymerase I 135 kDa polypeptide; DNA-directed RNA polymerase I 135kDa polypeptide; DNA-directed RNA polymerase I subunit RPA2; FLJ10816; FLJ21921; anti-POLR1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total POLR1Bprotein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1079

• Applicable Applications for anti-POLR1B antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from C-terminal of human POLR1B.
• Target Name: POLR1B
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from Jurkat/NIH-3T3 cells, using POLR1B antibody.)

Related Product Information for anti-POLR1B antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.Ota T., Nat. Genet. 36:40-45(2004).Hillier L.W., Nature 434:724-731(2005).Miller G., EMBO J. 20:1373-1382(2001).

Product Categories/Family for anti-POLR1B antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 212286172
• NCBI GeneID: 84172
• NCBI Accession #: NP_001131076.1
• NCBI GenBank Nucleotide #: NM_001137604.2
• UniProt Accession #: Q9H9Y6; Q2TAM4; Q585T5; Q6ZRR2; Q9H9D3; B7Z6Y7; B7Z823; F5GZX4; F8W898
• Molecular Weight: 107,858 Da
• NCBI Official Full Name: DNA-directed RNA polymerase I subunit RPA2 isoform 2
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide B, 128kDa
• NCBI Official Symbol: POLR1B
• NCBI Official Synonym Symbols: RPA2; RPA135; Rpo1-2
• NCBI Protein Information: DNA-directed RNA polymerase I subunit RPA2
• UniProt Protein Name: DNA-directed RNA polymerase I subunit RPA2
• UniProt Gene Name: POLR1B
• UniProt Synonym Gene Names: RNA polymerase I subunit 2; RPA135
• UniProt Entry Name: RPA2_HUMAN

NCBI Description

Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]

Uniprot Description

POLR1B: a subunit of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase that synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the clef. Two alternatively spliced human isoforms have been described.

Protein type: Transferase; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; EC 2.7.7.6; Nucleolus; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: nucleoplasm; cytoplasm; nucleolus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; ribonucleoside binding; metal ion binding

Biological Process: negative regulation of gene expression, epigenetic; nucleologenesis; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; embryo implantation; rRNA transcription