PTRF blocking peptide

PTRF Antibody (C-term) Blocking Peptide

• Gene Names: PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• Synonyms: PTRF; PTRF Antibody (C-term) Blocking Peptide; Polymerase I and transcript release factor; Cavin-1; PTRF blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human PTRF. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 390

• Cellular Location: Membrane, caveola. Cell membrane Microsome. Endoplasmic reticulum. Cytoplasm, cytosol. Mitochondrion. Nucleus Note: Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co- localizes with CAV1 in lipid rafts in adipocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PTRF blocking peptide

Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.

NCBI and Uniprot Product Information

• NCBI GI #: 56749614
• NCBI GeneID: 284119
• UniProt Accession #: Q6NZI2; O00535; Q6GMY1; Q96H74; Q9BT85; Q9HAP4; B2RAW7
• Molecular Weight: 22,597 Da
• NCBI Official Full Name: Polymerase I and transcript release factor
• NCBI Official Synonym Full Names: polymerase I and transcript release factor
• NCBI Official Symbol: PTRF
• NCBI Official Synonym Symbols: CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• NCBI Protein Information: polymerase I and transcript release factor
• UniProt Protein Name: Polymerase I and transcript release factor
• Protein Family: Polymerase I and transcript release factor
• UniProt Gene Name: PTRF
• UniProt Entry Name: PTRF_HUMAN

NCBI Description

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

Uniprot Description

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: caveola; cytoplasm; lipid raft; mitochondrion; nucleoplasm; nucleus; protein complex

Molecular Function: protein binding; rRNA primary transcript binding

Biological Process: termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Lipodystrophy, Congenital Generalized, Type 4