Rabbit PTRF Polyclonal Antibody | anti-PTRF antibody

Phospho-PTRF (Tyr156) Antibody

Cat. Num. AAA9614497

• Gene Names: PTRF; CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (90%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PTRF; Polyclonal Antibody; Phospho-PTRF (Tyr156) Antibody; 2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12; anti-PTRF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (90%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PTRF (Tyr156) Antibody detects endogenous levels of PTRF only when phosphorylated at Tyr156.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PTRF antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PTRF around the phosphorylation site of Tyr156.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Phosphorylation at Tyr-156 is essential for its functionin the regulation of ribosomal transcriptional activity.Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.Monoubiquitinated.
• Subunit Structure: Component of the CAVIN complex composed of CAVIN1, CAVIN2, CAVIN3 and CAVIN4. Homotrimer (By similarity). Interacts with TTF1. Interacts with RNA polymerase I. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148 (By similarity). Interacts with LIPE in the adipocyte cytoplasm. Interacts with CAV1 and CAVIN3 (By similarity). Interacts with CAVIN2. Interacts with CAVIN4 and CAV3.
• Similarity: The leucine-zipper domain 1 is essential for its localization in the caveolae.Belongs to the CAVIN family.
• Subcellular Location: Membrane>Caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm>Cytosol. Mitochondrion. Nucleus.; Note: Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using PTRF (Phospho-Tyr156) Antibody.Lane 1: Hela treated with blocking peptide.Lane 2: Hela;Lane 3: mouse lung;)

Western Blot (WB)

(Western blot analysis of PTRF (Phospho-Tyr156) using Serum treated COS7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-PTRF antibody

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 56749614
• NCBI GeneID: 284119
• UniProt Accession #: Q6NZI2; O00535; Q6GMY1; Q96H74; Q9BT85; Q9HAP4; B2RAW7
• Molecular Weight: 390
• NCBI Official Full Name: Polymerase I and transcript release factor
• NCBI Official Synonym Full Names: polymerase I and transcript release factor
• NCBI Official Symbol: PTRF
• NCBI Official Synonym Symbols: CGL4; CAVIN; CAVIN1; FKSG13; cavin-1
• NCBI Protein Information: polymerase I and transcript release factor; PTRF; TTF-I interacting peptide 12; RNA polymerase I and transcript release factor
• UniProt Protein Name: Polymerase I and transcript release factor
• Protein Family: Polymerase I and transcript release factor
• UniProt Gene Name: PTRF
• UniProt Entry Name: PTRF_HUMAN

NCBI Description

This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy. [provided by RefSeq, Nov 2009]

Uniprot Description

PTRF: Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex. Defects in PTRF are the cause of congenital generalized lipodystrophy type 4 (CGL4). It is a disorder characterized by the association of congenital generalized lipodystrophy with muscular dystrophy and cardiac anomalies. Congenital generalized lipodystrophy is characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the PTRF/SDPR family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: nucleoplasm; protein complex; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: protein binding; rRNA primary transcript binding

Biological Process: regulation of transcription, DNA-dependent; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter

Disease: Lipodystrophy, Congenital Generalized, Type 4