Ptk2b blocking peptide

Mouse Ptk2b Antibody (Center) Blocking Peptide

• Gene Names: Ptk2b; CAKB; FAK2; PYK2; CADTK; FADK2; Raftk; CAKbeta; E430023O05Rik
• Synonyms: Ptk2b; Mouse Ptk2b Antibody (Center) Blocking Peptide; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase; RAFTK; Fak2; Pyk2; Raftk; Ptk2b blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1009

• Cellular Location: Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cell cortex Nucleus. Note: Colocalizes with integrins at the cell periphery (By similarity). Interaction with NPHP1 induces the membrane-association of the kinase. Colocalizes with PXN at the microtubule-organizing center. The tyrosine phosphorylated form is detected at cell-cell contacts.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Ptk2b blocking peptide

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T- cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376' (By similarity). Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 408360096
• NCBI GeneID: 19229
• UniProt Accession #: Q9QVP9; B2RQ16; G3X8V1
• Molecular Weight: 115,794 Da
• NCBI Official Full Name: Protein-tyrosine kinase 2-beta
• NCBI Official Synonym Full Names: PTK2 protein tyrosine kinase 2 beta
• NCBI Official Symbol: Ptk2b
• NCBI Official Synonym Symbols: CAKB; FAK2; PYK2; CADTK; FADK2; Raftk; CAKbeta; E430023O05Rik
• NCBI Protein Information: protein-tyrosine kinase 2-beta
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Protein-tyrosine kinase
• UniProt Gene Name: Ptk2b
• UniProt Synonym Gene Names: Fak2; Pyk2; Raftk; CADTK; CAK-beta; CAKB; FADK 2; RAFTK
• UniProt Entry Name: FAK2_MOUSE

Uniprot Description

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; Nuclear receptor co-regulator; TK group; Fak family

Cellular Component: axon; cell cortex; cell soma; cytoplasm; dendrite; extrinsic to internal side of plasma membrane; focal adhesion; growth cone; lamellipodium; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; nucleus; perinuclear region of cytoplasm; postsynaptic density

Molecular Function: 3-phosphoinositide-dependent protein kinase binding; calmodulin-dependent protein kinase activity; enzyme binding; N-methyl-D-aspartate selective glutamate receptor activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein complex binding; protein kinase activity; receptor binding

Biological Process: actin filament organization; activation of JAK protein; angiogenesis; blood vessel endothelial cell migration; bone resorption; cell adhesion; cell surface receptor linked signal transduction; cellular defense response; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; focal adhesion formation; innate immune response; integrin-mediated signaling pathway; ionotropic glutamate receptor signaling pathway; MAPKKK cascade; marginal zone B cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of myeloid cell differentiation; negative regulation of neuron apoptosis; negative regulation of ossification; negative regulation of potassium ion transport; neurite development; oocyte maturation; peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of cellular protein metabolic process; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein kinase activity; positive regulation of synaptic transmission, glutamatergic; positive regulation of translation; protein amino acid phosphorylation; regulation of calcium-mediated signaling; regulation of cell adhesion; regulation of cell shape; regulation of cGMP biosynthetic process; regulation of inositol trisphosphate biosynthetic process; regulation of nitric oxide biosynthetic process; regulation of release of sequestered calcium ion into cytosol; response to hydrogen peroxide; sprouting angiogenesis; stress fiber formation; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway