Rabbit anti-Human LTBP-4 Polyclonal Antibody | anti-LTBP-4 antibody

LTBP-4 antibody

Cat. Num. AAA9402509

• Gene Names: LTBP4; ARCL1C; LTBP-4; LTBP4L; LTBP4S
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: LTBP-4; Polyclonal Antibody; LTBP-4 antibody; anti-LTBP-4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 1587

• Applicable Applications for anti-LTBP-4 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 64 and 256 of LTBP-4
• Target Name: LTBP-4
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with LTBP4 antibody [N1N2], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. The observed M.W. is based on the publication: PMID: 27148972)

Immunohistochemistry (IHC)

(LTBP4 antibody [N1N2], N-term detects LTBP4 protein at cytosol on human adrenal gland tumor by immunohistochemical analysis. Sample: Paraffin-embedded adrenal gland tumor. LTBP4 antibody [N1N2], N-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min)

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed A431, using LTBP4 antibody at 1: 200 dilution.)

SDS-PAGE (SDS)

(Sample (30 ug of whole cell lysate)A: HelaB: JurKat5% SDS PAGEPrimary antibody diluted at 1: 1000)

Related Product Information for anti-LTBP-4 antibody

Transforming growth factor-beta, or TGFB (see TGFB1; MIM 190180), remains biologically latent after secretion, blocked from activity by its N-terminal propeptide (the latency-associated peptide, or LAP). TGFB is activated by its dissociation from LAP. The latent form of TGFB is secreted in association with a latent TGFB-binding protein, or LTBP (see LTBP1; MIM 150390), and assembled into the extracellular matrix. LTBPs are mainly composed of epidermal growth factor (EGF; MIM 131530)-like repeats and protein domains with 8-cysteine repeats, both of which are also found in fibrillins (e.g., MIM 134797). See Oklu and Hesketh (2000) [PubMed 11104663] for a review of the LTBP gene family.[supplied by OMIM]

Product Categories/Family for anti-LTBP-4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 110347412
• NCBI GeneID: 8425
• NCBI Accession #: NP_003564
• NCBI GenBank Nucleotide #: NM_003573.2
• Molecular Weight: Predicted: 169kd
• NCBI Official Full Name: latent-transforming growth factor beta-binding protein 4 isoform b
• NCBI Official Synonym Full Names: latent transforming growth factor beta binding protein 4
• NCBI Official Symbol: LTBP4
• NCBI Official Synonym Symbols: ARCL1C; LTBP-4; LTBP4L; LTBP4S
• NCBI Protein Information: latent-transforming growth factor beta-binding protein 4
• UniProt Protein Name: Latent-transforming growth factor beta-binding protein 4
• UniProt Gene Name: LTBP4
• UniProt Synonym Gene Names: LTBP-4
• UniProt Entry Name: LTBP4_HUMAN

NCBI Description

The protein encoded by this gene binds transforming growth factor beta (TGFB) as it is secreted and targeted to the extracellular matrix. TGFB is biologically latent after secretion and insertion into the extracellular matrix, and sheds TGFB and other proteins upon activation. Defects in this gene may be a cause of cutis laxa and severe pulmonary, gastrointestinal, and urinary abnormalities. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

LTBP4: May be involved in the assembly, secretion and targeting of TGFB1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGFB1. May have a structural role in the extra cellular matrix (ECM). Defects in LTBP4 are the cause of Urban-Rifkin-Davis syndrome (URDS); also known as Cutis laxa with severe pulmonary gastrointestinal and urinary abnormalities. URDS is a syndrome characterized by disrupted pulmonary, gastrointestinal, urinary, musculoskeletal, craniofacial and dermal development. Clinical features include cutis laxa, mild cardiovascular lesions, respiratory distress with cystic and atelectatic changes in the lungs, and diverticulosis, tortuosity and stenosis at various levels of the intestinal tract. Craniofacial features include microretrognathia, flat midface, receding forehead and wide fontanelles. Belongs to the LTBP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: integrin binding; transforming growth factor beta receptor activity; protein binding; glycosaminoglycan binding; transforming growth factor beta binding; calcium ion binding

Biological Process: growth hormone secretion; extracellular matrix organization and biogenesis; regulation of transforming growth factor beta receptor signaling pathway; protein folding; regulation of cell differentiation; multicellular organismal development; transforming growth factor beta receptor signaling pathway; regulation of proteolysis; regulation of cell growth

Disease: Cutis Laxa, Autosomal Recessive, Type Ic