Mouse L1-Cell Adhesion Molecule (L1CAM) ELISA Kit | L1CAM elisa kit

Mouse L1-Cell Adhesion Molecule (L1CAM) ELISA Kit

Cat. Num. AAA2023953

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Reactivity: Mouse
• Synonyms: L1-Cell Adhesion Molecule (L1CAM); Mouse L1-Cell Adhesion Molecule (L1CAM) ELISA Kit; CD171; L1-CAM; CAML1; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; Antigen Identified By Monoclonal Antibody R1; L1CAM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of L1-Cell Adhesion Molecule (L1CAM).; No significant cross-reactivity or interference between L1-Cell Adhesion Molecule (L1CAM) and analogues was observed.
• Sequence Length: 1,248

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level L1-Cell Adhesion Molecule (L1CAM) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for L1CAM elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule (L1CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule (L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule (L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L1-Cell Adhesion Molecule (L1CAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for L1CAM elisa kit

CD & Adhesion molecule; Tumor immunity; Infection immunity; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 187951475
• NCBI GeneID: 3897
• UniProt Accession #: P32004; Q8TA87; A0AV65; A4ZYW4; B2RMU7; G3XAF4
• Molecular Weight: 138,908 Da
• NCBI Official Full Name: L1 cell adhesion molecule
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1
• UniProt Protein Name: Neural cell adhesion molecule L1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1
• UniProt Entry Name: L1CAM_HUMAN

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: presynaptic membrane; cell surface; focal adhesion; integral to membrane; plasma membrane; terminal button; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding

Biological Process: axon guidance; nervous system development; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; cell-cell adhesion mediated by integrin; homotypic cell-cell adhesion; positive regulation of cell-cell adhesion; homophilic cell adhesion; cell adhesion; blood coagulation; leukocyte migration

Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome