PGAP3 blocking peptide

PGAP3 Antibody (Center) Blocking peptide

• Gene Names: PGAP3; CAB2; PERLD1; PP1498; hCOS16; AGLA546
• Synonyms: PGAP3; PGAP3 Antibody (Center) Blocking peptide; Post-GPI attachment to proteins factor 3; COS16 homolog; hCOS16; Gene coamplified with ERBB2 protein; PER1-like domain-containing protein 1; CAB2; PERLD1; PGAP3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of PGAP3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 320

• Cellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Mainly localizes to Golgi apparatus.
• Tissue Location: Ubiquitously expressed, with highest levels in thyroid and placenta.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PGAP3 blocking peptide

Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable).

NCBI and Uniprot Product Information

• NCBI GI #: 74731724
• NCBI GeneID: 93210
• UniProt Accession #: Q96FM1; Q86Z03; Q8NBJ8; B4DGK7
• Molecular Weight: 33,967 Da
• NCBI Official Full Name: Post-GPI attachment to proteins factor 3
• NCBI Official Synonym Full Names: post-GPI attachment to proteins 3
• NCBI Official Symbol: PGAP3
• NCBI Official Synonym Symbols: CAB2; PERLD1; PP1498; hCOS16; AGLA546
• NCBI Protein Information: post-GPI attachment to proteins factor 3
• UniProt Protein Name: Post-GPI attachment to proteins factor 3
• Protein Family: Post-GPI attachment to proteins factor
• UniProt Gene Name: PGAP3
• UniProt Synonym Gene Names: CAB2; PERLD1; hCOS16
• UniProt Entry Name: PGAP3_HUMAN

NCBI Description

This gene encodes a glycosylphosphatidylinositol (GPI)-specific phospholipase that primarily localizes to the Golgi apparatus. This ubiquitously expressed gene is predicted to encode a seven-transmembrane protein that removes unsaturated fatty acids from the sn-2 position of GPI. The remodeling of the constituent fatty acids on GPI is thought to be important for the proper association between GPI-anchored proteins and lipid rafts. The tethering of proteins to plasma membranes via posttranslational GPI-anchoring is thought to play a role in protein sorting and trafficking. Mutations in this gene cause the autosomal recessive neurologic disorder hyperphosphatasia with mental retardation syndrome 4 (HPMRS4). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

PGAP3: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchors proteins. Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable). Belongs to the PGAP3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: intrinsic to endoplasmic reticulum membrane

Molecular Function: hydrolase activity, acting on ester bonds

Biological Process: GPI anchor metabolic process

Disease: Hyperphosphatasia With Mental Retardation Syndrome 4