PCAF recombinant protein

PCAF recombinant protein

Cat. Num. AAA515372

• Gene Names: KAT2B; P; CAF; PCAF; P/CAF
• Applications: Western Blot
• Synonyms: PCAF; PCAF recombinant protein; CAF; GCN5; GCN5L; P/CAF; GCN5L1; KAT2A; K(lysine) acetyltransferase 2B

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 4824

• Applicable Applications for PCAF recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant human PCAF (431-end) was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for PCAF recombinant protein

Recombinant human PCAF (431-end) was expressed in E. coli cells using a N-terminal GST tag.

Scientific Background: PCAF is a protein that shows homology to the yeast GCN5 and associates with p300/CBP. PCAF has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP (1). PCAF encodes a predicted 832-amino acid protein that showed by RNA blotting to be expressed in all tissues, most strongly in heart and skeletal muscle. PCAF has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. PCAF is required for MyoD activity and muscle differentiation. PCAF directly acetylated MyoD which then displays an increased affinity for its DNA target (2).

Product Categories/Family for PCAF recombinant protein

Acetyl/Methyltransferases Proteins; Signaling Proteins - Acetyl/Methyltransferase Proteins

References

1. Yang, X J. et al: A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Nature 382: 319-324, 1996.
2. Sartorelli, V. et al: Acetylation of MyoD directed by PCAF is necessary for the execution of the muscle program. Molec. Cell 4: 725-734, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 156071487
• NCBI GeneID: 8850
• NCBI Accession #: NP_003875.3
• NCBI GenBank Nucleotide #: NM_003884
• UniProt Accession #: Q92831; Q6NSK1
• Molecular Weight: ~69 kDa
• NCBI Official Full Name: Homo sapiens K(lysine) acetyltransferase 2B (KAT2B), mRNA
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 2B
• NCBI Official Symbol: KAT2B
• NCBI Official Synonym Symbols: P; CAF; PCAF; P/CAF
• NCBI Protein Information: histone acetyltransferase KAT2B; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF
• UniProt Protein Name: Histone acetyltransferase KAT2B
• UniProt Gene Name: KAT2B
• UniProt Synonym Gene Names: PCAF; Histone acetylase PCAF; P/CAF
• UniProt Entry Name: KAT2B_HUMAN

NCBI Description

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Ref.1 Ref.7 Ref.8 Ref.16

Catalytic activity: Acetyl-CoA + [histone] = CoA + acetyl-[histone]. Ref.4

Subunit structure: Interacts with SIRT1. Interacts (unsumoylated form) with NR2C1; the interaction promotes transactivation activity

By similarity. Interacts with EP300, CREBBP and DDX17. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Interacts with NR2C2 (hypophosphorylated and unsumoylated form); the interaction promotes the transactivation activity of NR2C2. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4. Interacts with MECOM. Interacts with E2F1; the interaction acetylates E2F1 augmenting its DNA-binding and transcriptional activity. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.19

Subcellular location: Nucleus

By similarity.

Tissue specificity: Ubiquitously expressed but most abundant in heart and skeletal muscle. Ref.1

Domain: The bromodomain mediates binding to HIV-1 Tat.

Sequence similarities: Belongs to the acetyltransferase family. GCN5 subfamily.Contains 1 bromo domain.Contains 1 N-acetyltransferase domain.