NSF blocking peptide

NSF Blocking Peptide

Cat. Num. AAA9624782

• Gene Names: NSF; SKD2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: NSF; NSF Blocking Peptide; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF_HUMAN; SKD 2; SKD2; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular-fusion protein NSF; NSF blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for NSF blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NSF blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 156564401
• NCBI GeneID: 4905
• NCBI Accession #: NP_006169.2
• NCBI GenBank Nucleotide #: NM_006178.3
• UniProt Accession #: P46459; Q8N6D7; Q9UKZ2; A8K2D9; B4DFA2
• Molecular Weight: 82,594 Da
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide-sensitive factor
• NCBI Official Symbol: NSF
• NCBI Official Synonym Symbols: SKD2
• NCBI Protein Information: vesicle-fusing ATPase; NEM-sensitive fusion protein; vesicular-fusion protein NSF; N-ethylmaleimide-sensitive fusion protein; N-ethylmaleimide-sensitive factor-like protein
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: NSFL1 cofactor
• UniProt Gene Name: NSF
• UniProt Synonym Gene Names: NEM-sensitive fusion protein
• UniProt Entry Name: NSF_HUMAN

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; Vesicle; Adaptor/scaffold; Protease; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: Golgi stack; lysosomal membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic shaft; cytosol

Molecular Function: protein binding; metal ion binding; ATPase activity; syntaxin-1 binding; protein complex binding; ATPase activity, coupled; protein kinase binding; Rab GTPase binding; ATP binding; PDZ domain binding

Biological Process: regulation of exocytosis; vesicle-mediated transport; intra-Golgi vesicle-mediated transport; synaptic transmission; exocytosis; metabolic process; Golgi to plasma membrane protein transport; Golgi vesicle docking; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion