Rabbit NSF Polyclonal Antibody | anti-NSF antibody

NSF Polyclonal Antibody

Cat. Num. AAA3015213

• Gene Names: NSF; SKD2
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep
• Applications: Western Blot
• Purity: affinity purified by Protein A
• Synonyms: NSF; Polyclonal Antibody; NSF Polyclonal Antibody; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF_HUMAN; SKD 2; SKD2; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular-fusion protein NSF; anti-NSF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep
• Clonality: Polyclonal
• Specificity: NSF Polyclonal Antibody detects endogenous levels of NSF protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-NSF antibody: Western Blot (WB)
• Application Notes: WB: 1:500-2000
• Immunogen: KLH conjugated synthetic peptide derived from human NSF
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

Related Product Information for anti-NSF antibody

Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the alpha-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the alpha-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the alpha-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).

NCBI and Uniprot Product Information

• NCBI GI #: 156564401
• NCBI GeneID: 4905
• NCBI Accession #: NP_006169.2
• NCBI GenBank Nucleotide #: NM_006178.3
• UniProt Accession #: P46459; Q8N6D7; Q9UKZ2; A8K2D9
• Molecular Weight: 82,594 Da
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide-sensitive factor
• NCBI Official Symbol: NSF
• NCBI Official Synonym Symbols: SKD2
• NCBI Protein Information: vesicle-fusing ATPase; NEM-sensitive fusion protein; vesicular-fusion protein NSF; N-ethylmaleimide-sensitive fusion protein; N-ethylmaleimide-sensitive factor-like protein
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: NSFL1 cofactor
• UniProt Gene Name: NSF
• UniProt Synonym Gene Names: NEM-sensitive fusion protein
• UniProt Entry Name: NSF_HUMAN

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; Vesicle; Adaptor/scaffold; Protease; EC 3.6.4.6

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: Golgi stack; lysosomal membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic shaft; cytosol

Molecular Function: protein binding; metal ion binding; ATPase activity; syntaxin-1 binding; protein complex binding; ATPase activity, coupled; protein kinase binding; Rab GTPase binding; ATP binding; PDZ domain binding

Biological Process: regulation of exocytosis; vesicle-mediated transport; intra-Golgi vesicle-mediated transport; synaptic transmission; exocytosis; metabolic process; Golgi to plasma membrane protein transport; Golgi vesicle docking; positive regulation of receptor recycling; potassium ion transport; plasma membrane fusion