Map2k1 blocking peptide

Mouse Map2k1 Antibody (C-term) Blocking peptide

• Gene Names: Map2k1; Mek1; MEKK1; MAPKK1; Prkmk1
• Synonyms: Map2k1; Mouse Map2k1 Antibody (C-term) Blocking peptide; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; Mek1; Prkmk1; Map2k1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of Mouse Map2k1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 393

• Cellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus Note: Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Map2k1 blocking peptide

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

NCBI and Uniprot Product Information

• NCBI GI #: 400275
• NCBI GeneID: 26395
• UniProt Accession #: P31938
• Molecular Weight: 43,474 Da
• NCBI Official Full Name: Dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: Map2k1
• NCBI Official Synonym Symbols: Mek1; MEKK1; MAPKK1; Prkmk1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: Map2k1
• UniProt Synonym Gene Names: Mek1; Prkmk1; MAP kinase kinase 1; MAPKK 1; MEK 1
• UniProt Entry Name: MP2K1_MOUSE

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family

Cellular Component: axon; cell cortex; cytoplasm; cytosol; dendrite; dendrite cytoplasm; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule; mitochondrion; nucleus; perikaryon; perinuclear region of cytoplasm; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine/tyrosine kinase activity; Ras GTPase binding; receptor signaling protein serine/threonine kinase activity; receptor signaling protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; Bergmann glial cell differentiation; cell cycle arrest; cell motility involved in cell locomotion; cell proliferation; cerebellar cortex formation; Golgi inheritance; heart development; keratinocyte differentiation; MAPKKK cascade; melanosome transport; mitosis; negative regulation of cell proliferation; negative regulation of homotypic cell-cell adhesion; neurite morphogenesis; neuron differentiation; positive regulation of axonogenesis; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of GTPase activity; positive regulation of Ras protein signal transduction; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; regulation of axon regeneration; regulation of protein amino acid phosphorylation; regulation of stress-activated MAPK cascade; regulation of vascular smooth muscle contraction; response to axon injury; response to glucocorticoid stimulus; response to oxidative stress; thymus development; thyroid gland development; vesicle transport along microtubule