HGS blocking peptide

HGS Antibody (N-term) Blocking Peptide

• Gene Names: HGS; HRS
• Synonyms: HGS; HGS Antibody (N-term) Blocking Peptide; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110; HRS; HGS blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human HGS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 777

• Cellular Location: Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane; Peripheral membrane protein
• Tissue Location: Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HGS blocking peptide

Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down- regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 71152119
• NCBI GeneID: 9146
• UniProt Accession #: O14964; Q9NR36
• Molecular Weight: 76,376 Da
• NCBI Official Full Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Synonym Full Names: hepatocyte growth factor-regulated tyrosine kinase substrate
• NCBI Official Symbol: HGS
• NCBI Official Synonym Symbols: HRS
• NCBI Protein Information: hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Protein Name: Hepatocyte growth factor-regulated tyrosine kinase substrate
• Protein Family: Hepatocyte growth factor-regulated tyrosine kinase substrate
• UniProt Gene Name: HGS
• UniProt Synonym Gene Names: HRS
• UniProt Entry Name: HGS_HUMAN

NCBI Description

The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]

Uniprot Description

Hrs: a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; cytosol; early endosome; endosome; intracellular membrane-bound organelle

Molecular Function: protein binding; protein domain specific binding

Biological Process: autophagy; endosome transport; membrane invagination; negative regulation of cell proliferation; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of JAK-STAT cascade; protein targeting to lysosome; regulation of MAP kinase activity; regulation of protein catabolic process; signal transduction