Hck blocking peptide

Mouse Hck Blocking Peptide (Center)

• Gene Names: Hck; Bmk; AI849071
• Synonyms: Hck; Mouse Hck Blocking Peptide (Center); Tyrosine-protein kinase HCK; B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK; Hck blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 239-252 of HUMAN Hck
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 524

• Cellular Location: Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol Isoform 2: Cell membrane; Lipid-anchor. Membrane, caveola; Lipid-anchor. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasmic vesicle. Lysosome. Nucleus Note: 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions (By similarity).
• Tissue Location: Expressed predominantly in cells of the myeloid and B-lymphoid lineages
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Hck blocking peptide

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 1170189
• NCBI GeneID: 15162
• UniProt Accession #: P08103; Q0VH03; Q3UD17
• Molecular Weight: 56,942 Da
• NCBI Official Full Name: Tyrosine-protein kinase HCK
• NCBI Official Synonym Full Names: hemopoietic cell kinase
• NCBI Official Symbol: Hck
• NCBI Official Synonym Symbols: Bmk; AI849071
• NCBI Protein Information: tyrosine-protein kinase HCK
• UniProt Protein Name: Tyrosine-protein kinase HCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Hck
• UniProt Synonym Gene Names: BMK
• UniProt Entry Name: HCK_MOUSE

NCBI Description

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010]

Uniprot Description

Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Src family

Cellular Component: actin filament; caveola; extrinsic to internal side of plasma membrane; focal adhesion; lysosome

Molecular Function: non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: cell differentiation; cytoskeleton organization and biogenesis; defense response to Gram-positive bacterium; innate immune response; negative regulation of apoptosis; peptidyl-tyrosine phosphorylation; phagocytosis; positive regulation of cell proliferation; protein amino acid autophosphorylation; regulation of cell proliferation; regulation of cell shape; regulation of phagocytosis; regulation of transcription factor activity; transmembrane receptor protein tyrosine kinase signaling pathway