Mouse anti-Human HCK Monoclonal Antibody | anti-HCK antibody

HCK Antibody

Cat. Num. AAA9215524

• Gene Names: HCK; JTK9; p59Hck; p61Hck
• Reactivity: Human
• Applications: Western Blot
• Synonyms: HCK; Monoclonal Antibody; HCK Antibody; Purified Mouse Monoclonal Antibody (Mab); Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck; anti-HCK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 1508CT602.13.1
• Specificity: This HCK antibody is generated from a mouse immunized with a recombinant protein of human HCK.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 505

• Applicable Applications for anti-HCK antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000-1:4000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-HCK Antibody at 1:2000-1:4000 dilutionLane 1: THP-1 whole cell lysatesLane 2: HL-60 whole cell lysatesLysates/proteins at 20 mug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-HCK antibody

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Product Categories/Family for anti-HCK antibody

Crown Antibodies; Immunology; Signal Transduction

References

Quintrell N.,et al.Mol. Cell. Biol. 7:2267-2275(1987). Ziegler S.F.,et al.Mol. Cell. Biol. 7:2276-2285(1987). Ota T.,et al.Nat. Genet. 36:40-45(2004). Deloukas P.,et al.Nature 414:865-871(2001). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 287326139
• NCBI GeneID: 3055
• NCBI Accession #: NP_001165600.1
• NCBI GenBank Nucleotide #: NM_001172129.1
• UniProt Accession #: P08631; Q29RX1; Q2VPE2; Q504R5; Q5T7K1; Q5T7K2; Q96CC0; Q9H5Y5; Q9NUA4; A8K1I1; B4DQB6; E1P5M2
• Molecular Weight: 59600
• NCBI Official Full Name: tyrosine-protein kinase HCK isoform b
• NCBI Official Synonym Full Names: HCK proto-oncogene, Src family tyrosine kinase
• NCBI Official Symbol: HCK
• NCBI Official Synonym Symbols: JTK9; p59Hck; p61Hck
• NCBI Protein Information: tyrosine-protein kinase HCK
• UniProt Protein Name: Tyrosine-protein kinase HCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: HCK
• UniProt Entry Name: HCK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

Uniprot Description

Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: Protein kinase, TK; EC 2.7.10.2; Oncoprotein; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 20q11-q12

Cellular Component: Golgi apparatus; transport vesicle; extrinsic to internal side of plasma membrane; focal adhesion; cell projection; lysosome; actin filament; caveola; nucleus; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; respiratory burst after phagocytosis; viral reproduction; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; regulation of phagocytosis; protein amino acid phosphorylation; regulation of defense response to virus by virus; regulation of cell shape; leukocyte migration during immune response; regulation of transcription factor activity; positive regulation of actin filament polymerization; leukocyte degranulation; regulation of inflammatory response; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; innate immune response; mesoderm development; innate immune response-activating signal transduction; inflammatory response; cell adhesion; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis