GRK6 blocking peptide

GRK6 Antibody (C-term) Blocking Peptide

• Gene Names: GRK6; GPRK6
• Synonyms: GRK6; GRK6 Antibody (C-term) Blocking Peptide; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; GPRK6; GRK6 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human GRK6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 576

• Cellular Location: Membrane; Lipid-anchor.
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GRK6 blocking peptide

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

NCBI and Uniprot Product Information

• NCBI GI #: 20141386
• NCBI GeneID: 2870
• UniProt Accession #: P43250; O60541; Q13652
• Molecular Weight: 64,294 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 6
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 6
• NCBI Official Symbol: GRK6
• NCBI Official Synonym Symbols: GPRK6
• NCBI Protein Information: G protein-coupled receptor kinase 6
• UniProt Protein Name: G protein-coupled receptor kinase 6
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK6
• UniProt Synonym Gene Names: GPRK6
• UniProt Entry Name: GRK6_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; Protein kinase, AGC; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: membrane; plasma membrane

Molecular Function: beta-adrenergic receptor kinase activity; protein binding

Biological Process: regulation of G-protein coupled receptor protein signaling pathway