Rabbit anti-Human, Rat G protein-coupled receptor kinase 6 Polyclonal Antibody | anti-GRK6 antibody

Rabbit anti-human G protein-coupled receptor kinase 6 polyclonal Antibody(GRK6)

Cat. Num. AAA1497037

• Gene Names: GRK6; GPRK6
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: G protein-coupled receptor kinase 6; Polyclonal Antibody; Rabbit anti-human G protein-coupled receptor kinase 6 polyclonal Antibody(GRK6); GRK6; G protein-coupled receptor kinase GRK6; GPRK6; anti-GRK6 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 560

• Applicable Applications for anti-GRK6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human G protein-coupled receptor kinase 6 protein (1-170aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-566 / sc-13080
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: G protein-coupled receptor kinase 6 antibody at 6ug/ml+rat heart tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 66,68,65 kDaObserved band size: 66 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1497037 at dilution 1:100)

Related Product Information for anti-GRK6 antibody

Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).

NCBI and Uniprot Product Information

• NCBI GI #: 51896037
• NCBI GeneID: 2870
• NCBI Accession #: NP_001004105.1
• NCBI GenBank Nucleotide #: NM_001004105.2
• UniProt Accession #: P43250; O60541; Q13652
• Molecular Weight: 64,294 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 6 isoform C
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 6
• NCBI Official Symbol: GRK6
• NCBI Official Synonym Symbols: GPRK6
• NCBI Protein Information: G protein-coupled receptor kinase 6
• UniProt Protein Name: G protein-coupled receptor kinase 6
• Protein Family: G protein-coupled receptor kinase
• UniProt Gene Name: GRK6
• UniProt Synonym Gene Names: GPRK6
• UniProt Entry Name: GRK6_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.16; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: cytosol; membrane; plasma membrane

Molecular Function: ATP binding; beta-adrenergic receptor kinase activity; G-protein coupled receptor kinase activity; protein binding

Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; negative regulation of anion channel activity; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; Wnt receptor signaling pathway