GNAS blocking peptide

GNAS Blocking Peptide

Cat. Num. AAA9626434

• Gene Names: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: GNAS; GNAS Blocking Peptide; Adenylate cyclase stimulating G alpha protein; AHO; C20orf45; Extra large alphas protein; GNAS complex locus; GNAS1; GPSA; GSA; GSP; Guanine nucleotide binding protein (G protein); alpha stimulating activity polypeptide 1; Guanine nucleotide binding protein G(s) alpha subunit; Guanine nucleotide binding protein G(s) subunit alpha isoforms XLas; guanine nucleotide regulatory protein; NESP; Neuroendocrine secretory protein; PHP1A; PHP1B; PHP1C; POH; RP4-543J19.4; Secretogranin VI; XLalphas; GNAS blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for GNAS blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GNAS blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4504047
• NCBI GeneID: 2778
• NCBI Accession #: NP_000507.1; NP_001070956.1; NP_001070957.1
• NCBI GenBank Nucleotide #: NM_000516.5
• UniProt Accession #: P63092; P04895; Q12927; Q14433; Q32P26; Q5JWD2; Q5JWD4; Q5JWD5; Q6NR75; Q6NXS0; A6NI00; E1P5G5; O95467; P84996; Q5JWF2
• Molecular Weight: 111,025 Da
• NCBI Official Full Name: protein GNAS isoform GNASL
• NCBI Official Synonym Full Names: GNAS complex locus
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; C20orf45
• NCBI Protein Information: protein ALEX; protein GNAS; protein SCG6 (secretogranin VI)
• UniProt Protein Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
• Protein Family: Protein
• UniProt Gene Name: GNAS
• UniProt Synonym Gene Names: GNAS1; GSP
• UniProt Entry Name: GNAS2_HUMAN

NCBI Description

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Uniprot Description

G-alpha(s): a guanine nucleotide-binding protein of the G12 class of G-alpha proteins. Agonist binding to Gq-coupled receptors may block Akt activation via the release of active G-alpha(q) subunits that inhibit phosphatidylinositol 3-kinase. Heterotrimeric G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein type: Oncoprotein; G protein; G protein, heterotrimeric alpha G(s); G protein, heterotrimeric; Vesicle; Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: dendrite; extracellular region; cytosol; ruffle; transport vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; heterotrimeric G-protein complex; trans-Golgi network membrane; intrinsic to membrane; nucleus

Molecular Function: GTPase activity; corticotropin-releasing hormone receptor 1 binding; ionotropic glutamate receptor binding; insulin-like growth factor receptor binding; signal transducer activity; protein binding; GTP binding; mu-type opioid receptor binding; metal ion binding; G-protein beta/gamma-subunit binding; beta-2 adrenergic receptor binding; D1 dopamine receptor binding; adenylate cyclase activity

Biological Process: developmental growth; positive regulation of osteoclast differentiation; water transport; protein secretion; female pregnancy; embryonic hindlimb morphogenesis; G-protein signaling, adenylate cyclase activating pathway; intracellular transport; positive regulation of cAMP biosynthetic process; DNA methylation; tissue homeostasis; transmembrane transport; endochondral ossification; post-embryonic body morphogenesis; dopamine receptor, adenylate cyclase activating pathway; response to drug; sensory perception of chemical stimulus; negative regulation of multicellular organism growth; multicellular organism growth; adenylate cyclase activation; sensory perception of smell; embryonic cranial skeleton morphogenesis; positive regulation of osteoblast differentiation; cartilage development; energy reserve metabolic process; renal water homeostasis; cAMP biosynthetic process; blood coagulation; cognition; regulation of insulin secretion

Disease: Osseous Heteroplasia, Progressive; Pseudohypoparathyroidism, Type Ic; Pseudopseudohypoparathyroidism; Mccune-albright Syndrome; Acth-independent Macronodular Adrenal Hyperplasia; Pseudohypoparathyroidism, Type Ia; Pseudohypoparathyroidism, Type Ib; Pituitary Adenoma, Growth Hormone-secreting