Epas1 blocking peptide

Mouse Epas1 Blocking Peptide (C-term)

• Gene Names: Epas1; HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
• Synonyms: Epas1; Mouse Epas1 Blocking Peptide (C-term); Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HLF; mHLF; HIF-related factor; HRF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Hif2a; Epas1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 713-727 of HUMAN Epas1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 874

• Cellular Location: Nucleus
• Tissue Location: Expressed in most tissues, with highest levels in lung, followed by heart, kidney, brain and liver. Predominantly expressed in endothelial cells. Also found in smooth muscle cells of the uterus, neurons, and brown adipose tissue. High expression in embryonic choroid plexus and kidney glomeruli
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Epas1 blocking peptide

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 3914285
• NCBI GeneID: 13819
• UniProt Accession #: P97481; O08787; O55046
• Molecular Weight: 96,712 Da
• NCBI Official Full Name: Endothelial PAS domain-containing protein 1
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: Epas1
• NCBI Official Synonym Symbols: HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
• NCBI Protein Information: endothelial PAS domain-containing protein 1
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• Protein Family: Endothelial PAS domain-containing protein
• UniProt Gene Name: Epas1
• UniProt Synonym Gene Names: Hif2a; EPAS-1; HLF; mHLF; HRF; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_MOUSE

Uniprot Description

HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: Transcription factor; DNA-binding

Cellular Component: cytoplasm; nuclear speck; nucleus; transcription factor complex

Molecular Function: cobalt ion binding; DNA binding; histone acetyltransferase binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: angiogenesis; blood vessel remodeling; cell differentiation; cell maturation; embryonic placenta development; erythrocyte differentiation; hemopoiesis; iron ion homeostasis; lung development; mitochondrion organization and biogenesis; myoblast cell fate commitment; negative regulation of transcription from RNA polymerase II promoter; norepinephrine biosynthetic process; norepinephrine metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of heart rate; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to hypoxia; response to oxidative stress; surfactant homeostasis; visual perception