Mouse anti-Human ENTPD8 Polyclonal Antibody | anti-Entpd8 antibody

ENTPD8 (Ectonucleoside Triphosphate Diphosphohydrolase 8, E-NTPDase 8, NTPDase 8, NTPDase8, UNQ2492/PRO5779)

Cat. Num. AAA647311

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ENTPD8; Polyclonal Antibody; ENTPD8 (Ectonucleoside Triphosphate Diphosphohydrolase 8; E-NTPDase 8; NTPDase 8; NTPDase8; UNQ2492/PRO5779); Anti -ENTPD8 (Ectonucleoside Triphosphate Diphosphohydrolase 8; anti-Entpd8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ENTPD8.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MGLSRKEQVFLALLGASGVSGLTALILLLVEATSVLLPTDIKFGIVFDAGSSHTSLFLYQWLANKENGTGVVSQALACQVEGPGISSYTSNAAQAGESLQGCLEEALVLIPEAQHRKTPTFLGATAGMRLLSRKNSSQARDIFAAVTQVLGRSPVDFWGAELLAGQAEGAFGWITVNYGLGTLVKYSFTGEWIQPPEEMLVGALDMGGASTQITFVPGGPILDKSTQADFRLYGSDYSVYTHSYLCFGRDQMLSRLLVGLVQSRPAALLRHPCYLSGYQTTLALGPLYESPCVHATPPLSLPQNLTVEGTGNPGACVSAIRELFNFSSCQGQEDCAFDGVYQPPLRGQFYVEASYPGQDRWLRDYCASGLYILTLLHEGYGFSEETWPSLEFRKQAGGVDIGWTLGYMLNLTGMIPADAPAQWRAESYGVWVAKVVFMVLALVAVVGAALVQLFWLQD

• Applicable Applications for anti-Entpd8 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human ENTPD8, aa1-458 (AAI46510.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ENTPD8 polyclonal antibody. Western Blot analysis of ENTPD8 expression in human pancreas.)

Western Blot (WB)

(Western Blot analysis of ENTPD8 expression in transfected 293T cell line by ENTPD8 polyclonal antibody. Lane 1: ENTPD8 transfected lysate (50.38kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-Entpd8 antibody

ENTPD8 is a canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. There are two different isoforms.

Product Categories/Family for anti-Entpd8 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 112821698
• NCBI GeneID: 72090
• NCBI Accession #: NP_082369.1
• NCBI GenBank Nucleotide #: NM_028093.1
• UniProt Accession #: Q8K0L2; Q6UQ22; A2AJ99
• Molecular Weight: 54,650 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 8
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 8
• NCBI Official Symbol: Entpd8
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 8; NTPDase8; NTPDase 8; E-NTPDase 8
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 8
• Protein Family: Ectonucleoside triphosphate diphosphohydrolase
• UniProt Gene Name: Entpd8
• UniProt Synonym Gene Names: E-NTPDase 8; NTPDase 8; NTPDase8
• UniProt Entry Name: ENTP8_MOUSE

Uniprot Description

Function: Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. Ref.1

Catalytic activity: A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate. Ref.1

Cofactor: Ca2+ or Mg2+. Has lower efficiency with Mg2+. Ref.1

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Expressed in liver, jejunum and kidney. Ref.1

Domain: The transmembranous domains are involved in regulation of enzyme activity

By similarity.

Post-translational modification: N-glycosylated

By similarity.

Sequence similarities: Belongs to the GDA1/CD39 NTPase family.

Biophysicochemical propertiesKinetic parameters:KM=13 µM for ATP Ref.1KM=41 µM for ADPKM=47 µM for UTPKM=171 µM for UDPpH dependence:Optimum pH is 5.5-8.0.