Rabbit anti-Mouse Epas1 Polyclonal Antibody | anti-Epas1 antibody

Mouse Epas1 Antibody (C-term)

Cat. Num. AAA9215863

• Gene Names: Epas1; HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: Epas1; Polyclonal Antibody; Mouse Epas1 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HLF; mHLF; HIF-related factor; HRF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Hif2a; anti-Epas1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This Mouse Epas1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 713-747 amino acids from the C-terminal region of Mouse Epas1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 874

• Applicable Applications for anti-Epas1 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Epas1 Antibody (C-term)at 1:2000 dilution + mouse heart lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 97 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Epas1 antibody

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).

Product Categories/Family for anti-Epas1 antibody

Cancer; Cardiovascular

References

Tian H.,et al.Genes Dev. 11:72-82(1997). Ema M.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:4273-4278(1997). Flamme I.,et al.Mech. Dev. 63:51-60(1997). Lando D.,et al.Genes Dev. 16:1466-1471(2002). Gradin K.,et al.J. Biol. Chem. 277:23508-23514(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 178057356
• NCBI GeneID: 13819
• NCBI Accession #: NP_034267.3
• NCBI GenBank Nucleotide #: NM_010137.3
• UniProt Accession #: P97481; O08787; O55046
• Molecular Weight: 96712
• NCBI Official Full Name: endothelial PAS domain-containing protein 1
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: Epas1
• NCBI Official Synonym Symbols: HLF; HRF; MOP2; HIF2A; bHLHe73; HIF-2alpha
• NCBI Protein Information: endothelial PAS domain-containing protein 1
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• Protein Family: Endothelial PAS domain-containing protein
• UniProt Gene Name: Epas1
• UniProt Synonym Gene Names: Hif2a; EPAS-1; HLF; mHLF; HRF; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_MOUSE

NCBI Description

HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: Transcription factor; DNA-binding

Cellular Component: transcription factor complex; cytoplasm; nuclear speck; nucleus

Molecular Function: protein dimerization activity; histone acetyltransferase binding; protein binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding; transcription factor activity; cobalt ion binding

Biological Process: mitochondrion organization and biogenesis; myoblast cell fate commitment; embryonic placenta development; transcription, DNA-dependent; regulation of heart rate; multicellular organismal development; cell maturation; negative regulation of transcription from RNA polymerase II promoter; norepinephrine metabolic process; iron ion homeostasis; visual perception; regulation of transcription, DNA-dependent; response to hypoxia; erythrocyte differentiation; hemopoiesis; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; response to oxidative stress; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell differentiation; surfactant homeostasis; lung development

Uniprot Description

HIF2A: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP. Interacts with EGLN1. Interacts with VHL. Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.

Protein type: DNA-binding; Transcription factor

Cellular Component: cytoplasm; nuclear speck; nucleus; transcription factor complex

Molecular Function: cobalt ion binding; DNA binding; histone acetyltransferase binding; protein binding; protein dimerization activity; protein heterodimerization activity; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: angiogenesis; blood vessel remodeling; cell differentiation; cell maturation; embryonic placenta development; erythrocyte differentiation; hemopoiesis; iron ion homeostasis; lung development; mitochondrion organization and biogenesis; multicellular organismal development; myoblast cell fate commitment; negative regulation of transcription from RNA polymerase II promoter; norepinephrine metabolic process; positive regulation of transcription from RNA polymerase II promoter; regulation of heart rate; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; regulation of transcription, DNA-dependent; response to hypoxia; response to oxidative stress; surfactant homeostasis; transcription, DNA-dependent; visual perception