DMTF1 blocking peptide

DMTF1 Antibody (C-term) Blocking Peptide

• Gene Names: DMTF1; DMP1; DMTF; MRUL; hDMP1
• Synonyms: DMTF1; DMTF1 Antibody (C-term) Blocking Peptide; Cyclin-D-binding Myb-like transcription factor 1; hDMTF1; Cyclin-D-interacting Myb-like protein 1; hDMP1; DMP1; DMTF1 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 760

• Cellular Location: Nucleus
• Tissue Location: Expressed at relatively low levels in colonic mucosa, ovary, peripheral leukocytes, prostate and small intestine, and at higher levels in spleen, testis and thymus Expressed in multiple regions of the brain and CNS including amygdala, caudate, corpus callosum, hippocampus, substantia nigra and subthalamic nucleus. Isoform 1 is the predominant isoform in monocytes, macrophages and neutrophils, isoform 2 is most strongly expressed in peripheral blood leukocytes and quiescent CD34 positive cells, and isoform 3 is expressed at low levels in all hematopoietic cell types. Expression is frequently reduced in non- small-cell lung carcinomas (NSCLC) due to hemizygous gene deletion, strongly suggesting that this locus is haploinsufficient for tumor suppression. Loss of this locus frequently occurs in tumors which retain wild-type CDKN2A/ARF and p53/TP53 loci Hemizygous gene deletion has also been observed in leukemic blasts from patients with abnormalities of the long arm of chromosome 7
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DMTF1 blocking peptide

Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity). Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity). Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.

NCBI and Uniprot Product Information

• NCBI GI #: 74762040
• NCBI GeneID: 9988
• UniProt Accession #: Q9Y222; Q05C48; Q59G79; Q6IS13; Q969T2; Q9H2Z2; Q9H2Z3; B2RBE1; B4DJS5
• Molecular Weight: 74,878 Da
• NCBI Official Full Name: Cyclin-D-binding Myb-like transcription factor 1
• NCBI Official Synonym Full Names: cyclin D binding myb like transcription factor 1
• NCBI Official Symbol: DMTF1
• NCBI Official Synonym Symbols: DMP1; DMTF; MRUL; hDMP1
• NCBI Protein Information: cyclin-D-binding Myb-like transcription factor 1
• UniProt Protein Name: Cyclin-D-binding Myb-like transcription factor 1
• Protein Family: Cyclin-D-binding Myb-like transcription factor
• UniProt Gene Name: DMTF1
• UniProt Synonym Gene Names: DMP1; hDMTF1; hDMP1
• UniProt Entry Name: DMTF1_HUMAN

NCBI Description

This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

DMTF1: Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest. Binds to the consensus sequence 5'-CCCG[GT]ATGT-3'. Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1. Belongs to the DMTF1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q21

Cellular Component: cytoplasm; nucleoplasm