Western Blot (WB)
(Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab.)
Rabbit anti-Human Hepatoma Derived Growth Factor Polyclonal Antibody | anti-HDGF antibody
Hepatoma Derived Growth Factor, CT (HDGF, DKFZp686J1764, FLJ96580, High-Mobility Group Protein 1-Like 2, HMG1L2, HMG-1L2) (AP)
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane). HDGF(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HDGF Antibody (C-term) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of HDGF Antibody (C-term) with SK-BR-3 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]
Uniprot Description
HDGF: Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor. Belongs to the HDGF family.
Protein type: Transcription regulation; Cytokine
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: nucleoplasm; extracellular space; transcriptional repressor complex; cytoplasm
Molecular Function: heparin binding; DNA binding; growth factor activity; nucleotide binding
Biological Process: cell proliferation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on HDGF
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Product Notes
The HDGF hdgf (Catalog #AAA6480046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hepatoma Derived Growth Factor, CT (HDGF, DKFZp686J1764, FLJ96580, High-Mobility Group Protein 1-Like 2, HMG1L2, HMG-1L2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatoma Derived Growth Factor can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-1:50 ELISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HDGF hdgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatoma Derived Growth Factor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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