C1orf77 blocking peptide

C1orf77 Blocking Peptide

Cat. Num. AAA9621572

• Gene Names: CHTOP; FOP; SRAG; SRAG-3; SRAG-5; pp7704; C1orf77; FL-SRAG
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: C1orf77; C1orf77 Blocking Peptide; chromatin target of chromatin target of PRMT1 protein; Chromatin target of PRMT1; Chromatin target of PRMT1 protein; Chromosome 1 open reading frame 77; CHTOP; CHTOP_HUMAN; DKFZp547E1010; FL- RP1-178F15.2; FL-SRAG; FOP; Friend of PRMT1; Friend of PRMT1 protein; HT031; MGC131924; MGC86949; OTTHUMP00000035122; OTTHUMP00000035123; Pp7704; RP1 178F15.2; Small arginine- and glycine-rich protein; Small protein rich in arginine and glycine; SRAG; SRAG-3; SRAG-5; C1orf77 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for C1orf77 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for C1orf77 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 28875797
• NCBI GeneID: 26097
• NCBI Accession #: NP_056422.2
• NCBI GenBank Nucleotide #: NP_056422.2
• UniProt Accession #: Q9Y3Y2; Q0VAQ8; Q2VPI9; Q5T7Y8; Q5T7Y9; Q5T7Z0; Q6NSM4; Q6PB28; Q8WYT9; Q9BUC5; Q9H034; D3DV55
• Molecular Weight: 21,918 Da
• NCBI Official Full Name: chromatin target of PRMT1 protein isoform 1
• NCBI Official Synonym Full Names: chromatin target of PRMT1
• NCBI Official Symbol: CHTOP
• NCBI Official Synonym Symbols: FOP; SRAG; SRAG-3; SRAG-5; pp7704; C1orf77; FL-SRAG
• NCBI Protein Information: chromatin target of PRMT1 protein
• UniProt Protein Name: Chromatin target of PRMT1 protein
• UniProt Gene Name: CHTOP
• UniProt Synonym Gene Names: C1orf77; FOP; SRAG
• UniProt Entry Name: CHTOP_HUMAN

NCBI Description

This gene encodes a small nuclear protein that is characterized by an arginine and glycine rich region. This protein may have an important role in the regulation of fetal globin gene expression and in the activation of estrogen-responsive genes. A recent study reported that this protein binds 5-hydroxymethylcytosine (5hmC) and associates with an arginine methyltransferase complex (methylosome), which promotes methylation of arginine 3 of histone H4 (H4R3) and activation of genes involved in glioblastomagenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

CHTOP: Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: intracellular membrane-bound organelle; nuclear speck; nucleolus; nucleoplasm

Molecular Function: methyl-CpG binding; protein binding

Biological Process: mRNA export from nucleus; positive regulation of ATPase activity; positive regulation of cell proliferation; positive regulation of helicase activity; positive regulation of histone methylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent