Interleukin 27 Receptor Alpha (IL27Ra) Recombinant Protein | IL27Ra recombinant protein

Recombinant Interleukin 27 Receptor Alpha (IL27Ra)

Cat. Num. AAA2018549

• Gene Names: Il27ra; CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 27 Receptor Alpha (IL27Ra); Recombinant Interleukin 27 Receptor Alpha (IL27Ra); IL27Ra recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LETQ MKPDTPQIFS QVDISEEATL EATVQWAPPV WPPQKVLICQ FRYKECQAET WTRLEPQLKT DGLTPVEMQN LEPGTCYQVS GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS GKRAALLVWK DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA
• Sequence Length: 623

• Applicable Applications for IL27Ra recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu117~Ala350 (Accession # O70394) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 170932522
• NCBI GeneID: 50931
• NCBI Accession #: NP_057880.2
• NCBI GenBank Nucleotide #: NM_016671.3
• UniProt Accession #: O70394; E9QNY4
• Molecular Weight: 29.8kDa
• NCBI Official Full Name: interleukin-27 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 27 receptor, alpha
• NCBI Official Symbol: Il27ra
• NCBI Official Synonym Symbols: CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
• NCBI Protein Information: interleukin-27 receptor subunit alpha; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; T cell cytokine receptor; IL-27 receptor subunit alpha; type I T-cell cytokine receptor; cytokine receptor family, class 1 (WSXWS), member 1; T cell cytokine receptor; cytokine receptor family, class 1 (WSXWS), member 1
• UniProt Protein Name: Interleukin-27 receptor subunit alpha
• Protein Family: Interleukin-27 receptor
• UniProt Gene Name: Il27ra
• UniProt Synonym Gene Names: Tccr; Wsx1; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; TCCR
• UniProt Entry Name: I27RA_MOUSE

Uniprot Description

IL27RA: Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1- type immune responses. Also appears to be involved in innate defense mechanisms. Belongs to the type I cytokine receptor family. Type 2 subfamily.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding; interleukin-27 receptor activity

Biological Process: positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; positive regulation of T-helper 1 type immune response; negative regulation of T-helper 2 type immune response; regulation of isotype switching to IgG isotypes