Phosph-Free Blocking Buffer
Phosph-Free Blocking Buffer, Synthetic
Synonyms
Phosph-Free Blocking Buffer; Synthetic; Phosph-Free Block ELISA Blocking Buffer; Phosph-Free Blocking Buffer blocking buffer
Form/Format
Supplied at 1X.
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
pH
7.4 at 1X
Contains
All synthetic materials, no phosphates.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Phosph-Free Blocking Buffer blocking buffer
Description: Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
Background: Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.
This block buffer is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Because this ELISA buffer contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. Use of this blocking formulation minimizes backgrounds without the use of conventional cross-reactive protein additives.
Phosph-Free Block works on all types of polystyrene plates except Immulon II microplates. When blocking with this buffer, recommends Corning 96-well EIA/RIA Stripwell microplates
Background: Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.
This block buffer is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Because this ELISA buffer contains inert non-reactive blockers, it reduces the amount of nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. Use of this blocking formulation minimizes backgrounds without the use of conventional cross-reactive protein additives.
Phosph-Free Block works on all types of polystyrene plates except Immulon II microplates. When blocking with this buffer, recommends Corning 96-well EIA/RIA Stripwell microplates
Product Categories/Family for Phosph-Free Blocking Buffer blocking buffer
Similar Products
Product Notes
The Phosph-Free Blocking Buffer (Catalog #AAA258389) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8-24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT's ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C. Researchers should empirically determine the suitability of the Phosph-Free Blocking Buffer for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosph-Free Blocking Buffer, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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