anti-Human STAT6 Antibody | anti-STAT6 antibody

STAT6 (Phospho-Thr645) Antibody

Cat. Num. AAA8510292

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: STAT6; STAT6 (Phospho-Thr645) Antibody; anti-STAT6 antibody

• Reactivity: Human
• Specificity: STAT6 (phospho-Thr645) antibody detects endogenous levels of STAT6 only when phosphorylated at threonine 645.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 847

• Applicable Applications for anti-STAT6 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of threonine 645 (P-A-TP-I-K).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT6 (phospho-thr645) antibody.)

References

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.

NCBI and Uniprot Product Information

• NCBI GI #: 296010864
• NCBI GeneID: 6778
• NCBI Accession #: NP_001171549.1
• NCBI GenBank Nucleotide #: NM_001178078.1
• UniProt Accession #: P42226; Q5FBW5; Q71UP4; A8K316; B7ZA27; F5GXI9
• Molecular Weight: 81,748 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6
• UniProt Protein Name: Signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator
• UniProt Gene Name: STAT6

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.