Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 Blocking Buffer
Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3
Synonyms
Blocking Buffer Optimization Pack: 100mL of BB1; BB2; BB3; Block Buffer Optimization Pack; BB3 blocking buffer
Form/Format
Supplied at 1X.
Application Notes
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
pH
7.4 at 1X
Contains
SynBlock-all synthetic materials; General Block-BSA; Neptune Block-non-mammalian proteins
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 blocking buffer
Description: Receive our three most popular Blocking Buffer formulations (General Block, Neptune Block, SynBlock) in this economical pack to evaluate the best blocking buffer for a particular assay.
Background: After coating the EIA/RIA plate with the capture antigen or antibody, proper blocking of the unoccupied areas of the plate wells is paramount to attaining an accurate signal. Assay sensitivity improves by utilizing the appropriate blocking buffer that effectively decreases background noise and improves the signal-to-noise ratio. Evaluation of blocking candidates is an important step of ELISA development. The Block Buffer Optimization Pack provides three of blocking buffer formulations for an economical and fast method of selecting the best blocking buffer for a particular assay. The Pack includes 100 mL of three ELISA blocking buffers, which is adequate to begin optimizing the blocking stage of the assay development process.
Background: After coating the EIA/RIA plate with the capture antigen or antibody, proper blocking of the unoccupied areas of the plate wells is paramount to attaining an accurate signal. Assay sensitivity improves by utilizing the appropriate blocking buffer that effectively decreases background noise and improves the signal-to-noise ratio. Evaluation of blocking candidates is an important step of ELISA development. The Block Buffer Optimization Pack provides three of blocking buffer formulations for an economical and fast method of selecting the best blocking buffer for a particular assay. The Pack includes 100 mL of three ELISA blocking buffers, which is adequate to begin optimizing the blocking stage of the assay development process.
Product Categories/Family for Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 blocking buffer
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Product Notes
The Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 (Catalog #AAA258062) is a Blocking Buffer and is intended for research purposes only. The product is available for immediate purchase. 1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8-24 hours at room temperature (RT). 3. Aspirate the coating solution. 4. Wash each well twice with ICT's ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C. Researchers should empirically determine the suitability of the Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Blocking Buffer Optimization Pack: 100mL of BB1, BB2, BB3, Blocking Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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