Isoguanosine- 5'- O- triphosphate (isoGTP) Biochemical

Isoguanosine- 5'- O- triphosphate (isoGTP), sodium salt

Cat. Num. AAA256656

• Purity: Typical purity is >95% (HPLC/ UV/ 292 nm) at time of quality control and packing. However, actual purity depends on storage and transport conditions. The product is not sterile and has not been tested for endotoxins.
• Synonyms: Isoguanosine- 5'- O- triphosphate (isoGTP); sodium salt; Isoguanosine- 5'- O- triphosphate (isoGTP) biochemical

• Purity/Purification: Typical purity is >95% (HPLC/ UV/ 292 nm) at time of quality control and packing. However, actual purity depends on storage and transport conditions. The product is not sterile and has not been tested for endotoxins.
• Form/Format: Tetrasodium. Aqueous solution of the sodium salt (10 mM).

• SMILES: O[C@H]1[C@@H](O)[C@H](N2C=NC3=C(NC(N=C32)=O)N)O[C@@H]1COP([O-])(OP(OP([O-])([O-])=O)([O-])=O)=O.[Na+].[Na+].[Na+].[Na+]
• CAS Number: 58083-93-5
• Formula: C??H??N?O??P?
• Properties: isoGTP can be useful in receptor mapping studies. The corresponding isoGDP is available as well.
• UV: lambda max 292 nm, epsilon 11100, pH 7
• Notes: Micromolar quantities are determined by UV at lambda max. When opening the tube please make sure that no liquid is lost within the cap. A short spin down in a bench centrifuge is recommended before use. ; Since triphosphates have multiple tasks in every organism, it is very likely that its analogues will interfere with many cell regulation processes in vivo. However, due to the rather small quantities to work with, no health hazards have been reported.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -70 degree C; It is most stable when stored as aqueous solution in the freezer (-20 degree Celsius necessary, -80 degree recommended), however, at ambient temperature the compound slowly starts to decompose. Thus, in order to maintain its original high quality it is recommended to allow thawing only before using the product. If you will not use up the vial with one application, please aliquot the contents of the vial in order to avoid repeated freeze/thaw cycles for the rest. When making such aliquots be sure to operate quickly and to freeze the vial again as soon as possible.; Shipping: Dry Ice

Structure

Related Product Information for Isoguanosine- 5'- O- triphosphate (isoGTP) biochemical

isoGTP is an analogue of adenosine-5'-O-triphosphate (ATP) with an additional carbonyl group at position 2 of thenucleobase and of guanosine-5'-O-triphosphate (GTP), respectively, where the carbonyl group at position 6 and the amino group atposition 2 have changed places.

References

Lascu, I.; Barzu, T.; Ty, N.G.; Ngoc, L.D.; Barzu, O.; Mantsch, H.H., Biochim. Biophys. Acta, 482, 251 - 260 (1977): "Regulatoryeffects of purine nucleotide analogs with liver glutamate dehydrogenase"
Weijland, A.; Sarfati, R.; Barzu, O.; Parmeggiani, A., FEBS Lett., 330, 334 - 338 (1993): "Asparagine?135 of elongation factor Tuis a crucial residue for the folding of the guanine nucleotide binding pocket"
Switzer, C.Y.; Moroney, S.E.; Benner, S.A., Biochemistry, 32, 10489 - 10496 (1993): "Enzymatic Recognition of the Base Pairbetween Isocytidine and Isoguanosine"
Despins, S.; Issur, M.; Bougie, I.; Bisaillon, M., Nucleic Acids Res., 38, 5493 - 5506 (2010): "Deciphering the molecular basis fornucleotide selection by the West Nile virus RNA helicase"
Attwater, J.; Tagami, S.; Kimoto, M.; Butler, K., Chem. Sci., 4, 2804 - 2814 (2013): "Chemical fidelity of an RNA polymeraseribozyme.