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Western Blot (WB) (TCEA1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TCEA1 antibody detected the TCEA1 protein (arrow).)

Rabbit anti-Human TCEA1 Polyclonal Antibody | anti-TCEA1 antibody

TCEA1 Antibody (Center)

Gene Names
TCEA1; SII; TCEA; TF2S; GTF2S; TFIIS
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TCEA1; Polyclonal Antibody; TCEA1 Antibody (Center); Transcription elongation factor A protein 1; Transcription elongation factor S-II protein 1; Transcription elongation factor TFIISo; GTF2S; TFIIS; anti-TCEA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TCEA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the Central region of human TCEA1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
98-126
Sequence Length
301
Applicable Applications for anti-TCEA1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(TCEA1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TCEA1 antibody detected the TCEA1 protein (arrow).)

Western Blot (WB) (TCEA1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TCEA1 antibody detected the TCEA1 protein (arrow).)

Immunohistochemistry (IHC)

(TCEA1 Antibody (Center) (MBS9205189)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCEA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (TCEA1 Antibody (Center) (MBS9205189)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCEA1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TCEA1 antibody
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
References
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Asp, J., et al. Genes Chromosomes Cancer 45(9):820-828(2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33970
NCBI Official Full Name
transcription elongation factor A protein 1 isoform 1
NCBI Official Synonym Full Names
transcription elongation factor A (SII), 1
NCBI Official Symbol
TCEA1
NCBI Official Synonym Symbols
SII; TCEA; TF2S; GTF2S; TFIIS
NCBI Protein Information
transcription elongation factor A protein 1
UniProt Protein Name
Transcription elongation factor A protein 1
UniProt Gene Name
TCEA1
UniProt Synonym Gene Names
GTF2S; TFIIS
UniProt Entry Name
TCEA1_HUMAN

Uniprot Description

TFIIS: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. Belongs to the TFS-II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 8q11.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; gene expression; DNA repair; regulation of RNA elongation

Research Articles on TCEA1

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Product Notes

The TCEA1 tcea1 (Catalog #AAA9205189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 98-126. The TCEA1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCEA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TCEA1 tcea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCEA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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