Western Blot (WB)
(Anti-MEP1A rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:70 kDa)
Rabbit anti-Mouse Meprin alpha/MEP1A Polyclonal Antibody | anti-MEP1A antibody
Anti-Meprin alpha/MEP1A Antibody, Rabbit Polyclonal
Gene Names
Mep1a; Mep1; Mep-1; Mep-1a; AI098089; AW107200
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Protein A
Synonyms
Meprin alpha/MEP1A; Polyclonal Antibody; Anti-Meprin alpha/MEP1A Antibody; Rabbit Polyclonal; MEP1A/Endopeptidase-2 Antibody; Rabbit PAb; meprin A; alpha (PABA peptide hydrolase); Anti-AI098089 Antibody; Anti-AW107200 Antibody; Anti-Mep-1 Antibody; Anti-Mep-1a Antibody; Anti-Mep1 Antibody; anti-MEP1A antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse Meprin alpha/MEP1A
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MEP1A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:5000-1:10,000
ELISA: 1:5000-1:10,000
Immunogen
Recombinant Mouse MEP1A protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse MEP1A (rM MEP1A). Total IgG was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-MEP1A rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:70 kDa)
Western Blot (WB)
(Anti-MEP1A rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:70 kDa)
Related Product Information for anti-MEP1A antibody
Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein that belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo-and hetero-oligomeric enzymes. Meprins are abundantly expressed in the kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials, and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury (AKI).
References
Bertenshaw, GP. et al., 2002, Biol Chem. 383 (7-8):1175-83. Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22. Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18. Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,231 Da
NCBI Official Full Name
meprin A subunit alpha
NCBI Official Synonym Full Names
meprin 1 alpha
NCBI Official Symbol
Mep1a
NCBI Official Synonym Symbols
Mep1; Mep-1; Mep-1a; AI098089; AW107200
NCBI Protein Information
meprin A subunit alpha; meprin alpha; endopeptidase-2; meprin A alpha-subunit
UniProt Protein Name
Meprin A subunit alpha
Protein Family
UniProt Gene Name
Mep1a
UniProt Entry Name
MEP1A_MOUSE
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Product Notes
The MEP1A mep1a (Catalog #AAA8110874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Meprin alpha/MEP1A Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Meprin alpha/MEP1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000. Researchers should empirically determine the suitability of the MEP1A mep1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Meprin alpha/MEP1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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