Testing Data
BCR-RET recombinant protein
BCR-RET Mutant, Active
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Testing Data
Testing Data
Testing Data #2
Testing Data #2
Scientific Background: BCR-RET is a chimaeric fusion of BCR and RET genes generated by a balanced translocation of genes from t (10;22) (q11;q11) and this leads to aberrant activation of RET activity which can transform hematopoietic cells and skew the hematopoietic differentiation program towards the monocytic/macrophage lineage (1). The BCR-RET fusion is frequently found in chronic myelomonocytic leukemia (CMML) cases. The RET fusion genes seem to constitutively mimic the same signaling pathway as RAS mutations frequently involved in CMML.
NCBI and Uniprot Product Information
NCBI Description
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Bcr: a protein with serine/threonine protein kinase activity and is a GTPase-activating protein (GAP) for RAC1 and CDC42. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. A breakpoint cluster region protein that participates in a t(9;22)(q34;q11) chromosomal translocation that produces a BCR-ABL oncogene responsible for chronic myeloid leukemia (CML), acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Tyr177 of human Bcr, phosphorylated in the Bcr-Abl fusion protein, provides a docking site for Gab2 and GRB2, and is important in the transforming activity of Bcr-Abl. Sequence variants of the Bcr protein may be associated with bipolar disorder. Two alternatively spliced isoforms of the human protein have been reported.
Protein type: ATYPICAL group; BCR family; EC 2.7.11.1; GAP; GAP, Rac/Rho; GEF, Rac/Rho; Kinase, protein; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical
Chromosomal Location of Human Ortholog: 22q11.23
Cellular Component: cytosol; membrane; postsynaptic density; protein complex
Molecular Function: GTPase activator activity; kinase activity; protein binding; protein-tyrosine kinase activity
Biological Process: positive regulation of GTPase activity; protein amino acid phosphorylation; regulation of small GTPase mediated signal transduction; signal transduction
Disease: Leukemia, Acute Lymphoblastic; Leukemia, Chronic Myeloid
Research Articles on BCR-RET
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Product Notes
The BCR-RET bcr (Catalog #AAA515646) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-426 (BCR) - 713-end (RET). AAA Biotech's BCR-RET can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the BCR-RET bcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCR-RET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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