anti-Human Cathepsin D Polyclonal Antibody | anti-CTSD antibody

Anti-Cathepsin D Antibody

Cat. Num. AAA177659

• Gene Names: CTSD; CPSD; CLN10; HEL-S-130P
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Cathepsin D; Polyclonal Antibody; Anti-Cathepsin D Antibody; CatD; CATD_HUMAN; Cathepsin D heavy chain; Cathepsin D light chain; CathepsinD; CD; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; CTSD; Lysosomal aspartyl peptidase; Lysosomal aspartyl protease; MGC2311; cathepsin D; anti-CTSD antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 412

• Applicable Applications for anti-CTSD antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human Cathepsin D recombinant protein (Position: M201-L412). Human Cathepsin D shares 84.4% and 84% amino acid (aa) sequence identity with mouse and rat Cathepsin D, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of Cathepsin D expression in HEPG2 whole cell lysates (lane 1), A549 whole cell lysates (lane 2) and PANC whole cell lysates (lane 3). Cathepsin D at 28KD was detected using rabbit anti- Cathepsin D Antigen Affinity purified polyclonal antibody at0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(Cathepsin D was detected in paraffin-embedded sections of human Intestinal cancer tissues using rabbit anti- Cathepsin D Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-CTSD antibody

Description: Rabbit IgG polyclonal antibody for Cathepsin D(CTSD) detection. Tested with WB, IHC-P in Human.

Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

References

1. Faust PL, Kornfeld S, Chirgwin JM (Sep 1985). "Cloning and sequence analysis of cDNA for human cathepsin D". Proc Natl Acad Sci U S A 82 (15): 4910-4. 2. Wolf M, Clark-Lewis I, Buri C, Langen H, Lis M, Mazzucchelli L (April 2003). "Cathepsin D specifically cleaves the chemokines macrophage inflammatory protein-1 alpha, macrophage inflammatory protein-1 beta, and SLC that are expressed in human breast cancer". Am. J. Pathol. 162 (4): 1183-90.

NCBI and Uniprot Product Information

• NCBI GI #: 4503143
• NCBI GeneID: 1509
• NCBI Accession #: NP_001900.1
• NCBI GenBank Nucleotide #: NM_001909.4
• UniProt Accession #: P07339; Q6IB57
• Molecular Weight: 44,552 Da
• NCBI Official Full Name: cathepsin D preproprotein
• NCBI Official Synonym Full Names: cathepsin D
• NCBI Official Symbol: CTSD
• NCBI Official Synonym Symbols: CPSD; CLN10; HEL-S-130P
• NCBI Protein Information: cathepsin D
• UniProt Protein Name: Cathepsin D
• Protein Family: Cathepsin
• UniProt Gene Name: CTSD
• UniProt Synonym Gene Names: CPSD
• UniProt Entry Name: CATD_HUMAN

NCBI Description

This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSD: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. Consists of a light chain and a heavy chain. Belongs to the peptidase A1 family.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.23.5; Autophagy

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular matrix; extracellular region; extracellular space; lipid raft; lysosomal lumen; lysosome; melanosome

Molecular Function: aspartic-type endopeptidase activity; cysteine-type endopeptidase activity; protein binding; serine-type endopeptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; autophagy; collagen catabolic process; protein catabolic process; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 10