Bcl-2-like protein 1 (Bcl2l1) Recombinant Protein | Bcl2l1 recombinant protein

Recombinant Rat Bcl-2-like protein 1 (Bcl2l1)

• Gene Names: Bcl2l1; Bclx; Bcl2l; bcl-X; Bcl-xl
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Bcl-2-like protein 1 (Bcl2l1); Recombinant Rat Bcl-2-like protein 1 (Bcl2l1); Recombinant Bcl-2-like protein 1 (Bcl2l1); Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; Bcl2l1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-233
• Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEPERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
• Sequence Length: 233

• Species: Rattus norvegicus (Rat)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 75992930
• NCBI GeneID: 24888
• NCBI Accession #: NP_001028842.1
• NCBI GenBank Nucleotide #: NM_001033670.1
• UniProt Accession #: P53563; P70613; P70614; Q52KS0; Q62678; Q62836; Q64087; Q64128
• Molecular Weight: 26,158 Da
• NCBI Official Full Name: bcl-2-like protein 1 isoform 2
• NCBI Official Synonym Full Names: Bcl2-like 1
• NCBI Official Symbol: Bcl2l1
• NCBI Official Synonym Symbols: Bclx; Bcl2l; bcl-X; Bcl-xl
• NCBI Protein Information: bcl-2-like protein 1; bcl2-L-1; B cell lymphoma 2 like; B cell lymphoma like X; apoptosis regulator Bcl-X
• UniProt Protein Name: Bcl-2-like protein 1
• Protein Family: Bcl-2-like protein
• UniProt Gene Name: Bcl2l1
• UniProt Synonym Gene Names: Bclx; Blc2l; Bcl2-L-1
• UniProt Entry Name: B2CL1_RAT

NCBI Description

inhibits neuronal apoptosis; may provide neuroprotection against ischemic brian injury [RGD, Feb 2006]

Uniprot Description

Function: Potent inhibitor of cell death. Inhibits activation of caspases

By similarity. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis. Ref.3 Ref.4 Ref.6

Subunit structure: Homodimer

By similarity. Isoform Bcl-X(L) forms heterodimers with BAX, BAK or BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Interacts with BAD and BBC3. Interacts (isoform Bcl-X(L)) with SIVA1 (isoform 1); the interaction inhibits the anti-apoptotic activity. Interacts with BECN1 and PGAM5. Interacts (isoform Bcl-X(L)) with BAX (isoform Sigma). Interacts with BCL2L11

By similarity. Interacts with DMN1L; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Isoform Bcl-X(L) interacts with IKZF3

By similarity. Interacts with HEBP2

By similarity. Ref.6

Subcellular location: Mitochondrion membrane; Single-pass membrane protein

By similarity. Nucleus membrane; Single-pass membrane protein; Cytoplasmic side

By similarity. Cytoplasm › cytoskeleton › centrosome

By similarity. Note: Mitochondrial membranes and perinuclear envelope

By similarity.

Tissue specificity: Expressed in most tissues. Bcl-X(beta) is specifically expressed in cerebellum, heart, and thymus. In the ovary, the predominant form is Bcl-X(L), with a small but detectable level of Bcl-X(S).

Domain: The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.

Post-translational modification: Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity

By similarity.Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis

By similarity.

Sequence similarities: Belongs to the Bcl-2 family.

Sequence caution: The sequence AAC60701.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAC60702.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.