BCHE / Cholinesterase Recombinant Protein | BCHE recombinant protein

Human BCHE / Cholinesterase Recombinant Protein

• Gene Names: BCHE; E1; CHE1; CHE2; BCHED
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: BCHE / Cholinesterase; Human BCHE / Cholinesterase Recombinant Protein; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; CHE1; BCHE recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 602

• Applicable Applications for BCHE recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Homotetramer; disulfide-linked. Dimer of dimers.
• Entry Function: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 116353
• NCBI GeneID: 590
• UniProt Accession #: P06276
• NCBI Official Full Name: Cholinesterase
• NCBI Official Synonym Full Names: butyrylcholinesterase
• NCBI Official Symbol: BCHE
• NCBI Official Synonym Symbols: E1; CHE1; CHE2; BCHED
• NCBI Protein Information: cholinesterase
• UniProt Protein Name: Cholinesterase
• Protein Family: Cholinesterase
• UniProt Gene Name: BCHE
• UniProt Synonym Gene Names: CHE1
• UniProt Entry Name: CHLE_HUMAN

NCBI Description

This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]

Uniprot Description

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: Secreted, signal peptide; Hydrolase; Nuclear envelope; Secreted; EC 3.1.1.8

Chromosomal Location of Human Ortholog: 3q26.1-q26.2

Cellular Component: membrane; nuclear envelope lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: identical protein binding; choline binding; enzyme binding; cholinesterase activity; beta-amyloid binding; acetylcholinesterase activity; catalytic activity

Biological Process: response to drug; response to alkaloid; negative regulation of cell proliferation; choline metabolic process; cocaine metabolic process; cellular protein metabolic process; response to folic acid; response to glucocorticoid stimulus; neuroblast differentiation; learning; negative regulation of synaptic transmission